The Effect Of Yuxianling Granula On The Learning And Memory, Hippocampus Mfs Of Ka Induced Temporal Lobe Epilepsy In Rat

[Objective] To observe the effect of Yuxianling Granula on the learning and memory, hippocampus MFS(mossy fiber sprouting) of KA induced-temporal lobe epilepsy in rat,and to study the impact of the related mechanisms.[Methods] By stereotactic apparatus, we locally injected KA in hippocampus of rats,and established model of temporal lobe epilepsy, Induced seizures. To ev-aluate the effct of Yuxianling Granula on the learning and memory ability in th-e epileptic rats by the performance of learning and memory in rat water maze test; Using transmission electron microscopy to observe the changes of ultrastructural in hippocampal CA3 area; Using silver nitrate staining Timm to observe hippoca-mpal mossy fiber sprouting situation; To detect the change of the growth associa-ted protein (GAP43),which is markers of mossy fiber sprouting,by Immuno histo-chemical method,in order to exploer the effect of Yuxianling Granula on the lear-ning and memory,hippocampus MFS(mossy fiber sprouting) of KA induced tem-poral lobe epilepsy in rat,and to study the impact of the related mechanisms.[Results] 1.a water maze test resultsLearning and memory of Yuxianling Granula group and lamotrigine group improved significantly, compared with the control group, learning, memory per- formance was no significant difference (P> 0.05), compared with model group, learning and memory performance differences was statistically significant (P< 0.05); Comparation between Yuxianling Granula group and lamotrigine groups showed no significant difference (P> 0.05), it prompted that Yuxianling Granula, lamotrigine can both significantly improve learning and memory in epilepsy mo-del rats.2.Results of ultrastructure in hippocampal CA3 by TEMIn the control group, nuclei in CA3 neurons in hippocampus were round, m-embrane structure was clear, there were mitochondria, rough endoplasmic reticu-lum, smooth endoplasmic reticulum and lots of ribosomes in cytoplasm. In Sha-m group, we could see focal edema in the vicinity of glial cells. In Model group, the nuclear membrane was shrinkage, Axis reduced degeneration, Cytoplasmic o-rganelles is unclear, Heterochromatin increased margination obvious, borders we-re clearly. A wide range of nearby neurons was edema. Parts of glial cells was ed-ema obviously. Endothelial cell was shrinkage, apoptosis-like changes.Neurons and neuropil was within the lower density of synaptic activity. Lamotrigine group of neurons in the membrane slightly wrinkled, local small amount of edema. Nu-cleolus in Yuxianling Granula was clear, structure in the cell was clear. Tips Yu-xianling Granula, lamotrigine can antagonize hippocampus CA3 area ultrastr-ucture injury of KA induced temporal lobe epilepsy.3. Results of hippocampal CA3 area by Timm staining mossy fiber spr-outing MOD and IOD Yuxianling Granula group and lamotrigine group decreased obvioursly;Co-mpara with the control group and sham operation group,MOD and IOD was sign-ificantly (P<0.05); with model group, the difference between MOD and IOD was statistically significant (P<0.05); Comparation between Yuxianling Granula gro-up and the lamotrigine group, the differences between MOD and IOD were stat-istically significant (P< 0.05), It suggested that Yuxianling Granula can signiic-antly reduce the overall positive degree in hippocampal CA3 area MFS of KA i-nduced temporal lobe epilepsy, and the improving their positive effect in Yuxian ling Granula group was more clearly than that in Lamotriazine group.4. Results of Immunohistochemical detection of mossy fiber sprouting of nerve growth associated protein markers (GAP-43)Growth associated protein MOD and IOD in Yuxianling Granula group,La-motrigine group decreased, compared with the control group, differences betwen MOD and IOD in both group were statistically significant (P<0.05); compared with the sham group, the difference was not statistically significant (P> 0.05); co-mpared with model group, the differences were statistically signi-ficant (P<0.01); compared Yuxianling Granula group with the lamotrigine group, the difference w as statistically significant (P<0.05),It prompted Yuxianling Granula can inhibit t-he expression of the KA induced temporal lobe epilepsy rat hippocampal CA3 ar-ea GAP-43 protein, and more effectively than lamotrigine.[Conclusion]1. KA induced temporal lobe epilepsy rats can learn and memory. 2. Yuxianling Granula, lamotrigine can improve the learning and memory ability of KA induced temporal lobe epilepsy rat.3.The mechanism of Yuxianling Granula improving learning and memory of KA induced temporal lobe epilepsy rats may be related to its reduce in the over-Ex pression of hippocampal CA3 area GAP-43immune response product, there-by inhibiting hippocampal abnormal mossy fiber sprouting, and thus protect the hippocampal neurons.