Effects Of Moxibustion On Signal Molecule Nos1,nos3 Associated With Erectile Function In Erectile Dysfunction Rats With Disabetes Mellitus

Objective:To study the effect of moxibustion in erectile function, the level of blood glucose, the activity of NOSl and NOS3 in Erectile Dysfunction Rats with Diabetes Mellitus.Method:110 Male Sprague-Dawley rats with normal Erectile function were injected with streptozotozin (STZ) to make experimental models of diabetes mellitus (DM). then injected with apomorphine in all of the DM rats to select erectile dysfunction rats models with diabetes. The normal 10 rats is the Normal group, the ED rats were devided into Model group and Moxibustion group in random. The Moxibustion group received Moxibustion on acupoint "Shenshu" (BL23) and "Sanyinjiao" (SP6). Examine the change of Erectile of the three groups after the treatment. Detect the change of blood sugar using blood -sugar detection. Immunohistochemistry and In situ hybridinzation was used to study the expression of NOS1 and NOS3 in the penis.Results:1.Comparing with model group, the blood sugar level of moxibustion group is low to some extent. The difference has statistical significance. (P<0.01);2.The counts of rats which had the ability of erection (P<0.05), the erection rate (P<0.05) and the total frequency of erection (P>0.05) in the model-moxibustion group were superior to the model group after the treatment. And the counts of rats which had the ability of erection, the erection rate and the total frequency of erection in the model-moxibustion and the model group were inferior to the blank group (P<0.01);3. The expression of NOS1 and NOS3 in the penis measured with immunohistochemistry technique reduced significantly in the moxibustion group and the model group, compared with that in the normal group (P=0.00<0.05) after the treatment. And the expression of NOS1 and NOS3 in the moxibustion group was much higher than that in the model group (P=0.00<0.05);4. The expression of NOS1 and NOS3 in the penis measured with In situ hybridinzation technique reduced significantly in the moxibustion group and the model group, compared with that in the normal group (P=0.00<0.05) after the treatment. And the expression of NOS1 and NOS3 in the moxibustion group was much higher than that in the model group (P=0.00<0.05)Conclusion:The results show that moxibustion decreases the level of blood sugar, and the level of NOS1 and NSO3 in the penis. Moxibustion has some effects in promoting the Erectile function of Diabetic rats.