The Influnce Of Yinqiao San To β-defensin1 Gene Expression Of Model Mouse Lung Tissue Infectted By Flu Virus

Objective To discuss the influence of YinQiaoSan toβ-defensinl gene expression of model mouse lung tissue infected by flu virus. Partly post the immunity charge mechanism of anti-virus by Chinese Medicine compound.Lend experiment basis for clinic application of YinQiaoSan preventing and curing flu virusMethods (1) Influenza virus are Passage and determined its Virulence. Influenza H1N1 virus infection 9 day-old vaccinations atlantics cavity nest in chicken embryos, red blood clots measured for Virulence. (2) mice are infected by influenza virus. Mice are infected with different concentrations of influenza virus when they are anesthetized by a ether. intranasal mice were LD50. Detecting their LD50 (3) mice divide into groups and treat. Mice randomly divided into Chinese traditional medicine group, the western medicine group, the model group. In the ether 4LD50 infection in drugged, two days before the mice infected, TCM group to begin with YinQiaoSan scattered 60g/kg/d gastric larvae, Western medicine group to bingDuZuo 0.07 g/kg/d intraperitoneal injection, The model group releases physiological saline, d2, larvae d4 group, Chinese traditional medicine group and the group d8 western groups of mice were in the first 2 days after infection,4 days, the first eight days of death, lung homogenate extraction using Real time-RNA polymerase chain reaction (PCR) detecting group RT-mouse lung tissue DEFB1 expression. Experimental results with standard deviation followed by SPSS10.0 test statistics processing, analysis the differences between the groups.Results (1) After hand from generation to generation. The influenza H1N1 virus have minimum virus degrees is 1:640. fitting the require for doing experiment by animal. (2) Mice get ill after be infected by influenza virus, there are some symptoms appeared in mice, unbalanced less active, without burnish, weight, reduce appetite, mostly in the gradual failure,7 days began to death. LD50=6.5, namely, a virus levitation liquid dilution into 10-6.5, can make the 50% of mice death after inoculated 0.1 ml of virus. (3) The only a single gene amplification product, product expansion with peak. M-DEFB1 and M-ACTB genetic gene have the size:195 bp and 111bp.The length of each gene fragments with consistent design, length of the genes we test for our product expansion to the testing purpose gene product. (4)Western medicine and traditional medicine group, both of their mBDF1 expression are high model group, and the quantity of significant difference,; Western medicine group, the group BDF1mRNA expression in after infection 1-8 days are presented first, then decreased, and the changes in the amount BDF1mRNA expression appears four days, before each time point is a significant change point in time. (5)The first two days after infection, The mBDF1(22.74±1.1.410) of traditional medicine group more higher than western medicine group (21.1.0±1.531).But when Eight Days, western group (22.76±0.783) higher than traditional medicine group (20.20±11.367)Conclusions (1) The experiment results show that the YinQiao San. Scattered in the influenza virus infection, early significantly increased BDF1mRNA lung tissue, YinQiaoSan is expressed in the initial play natural infection immune defenses.(2)YinqiaoSan anti-infection and immune regulation may DEFB1 up regulation, which antiviral mechanism and regulation of the immune response.