Clinical Significance Of Detection In The Serum And Csf Levels Of Sdf-1 In Patients With Acute Leukemia

Objective:To detect the alterations in the serum and cerebrospinal fiuid (CSF) levels of stromal cell derived factor-1 (SDF-1) in patients with acute leukemia (AL) and study the clinical significance.Method:45 case patients of acute leukemia were studied, include 26 case AML,19 case ALL,4 case final diagnosis central nervous system leukemia (CNSL). All the patients were taken serum specimen before chemotherapy and 40 case were taken CSF before intrathecal injection. With enzyme-linked immunoabsorbent assay (ELISA), the level of SDF-1 in the peripheral blood of 45 patients and in the CSF of 40 patients were measured, and 20 case normal-control subjects also were detected as comparison, and some other related items were determined as well. To compare the variation level of SDF-1 in the serum and CSF with different acute leukemia patients and observe other related item. All data were analyzed by SPSS 14.0.Results:1. The average plasma level of SDF-1 in the ALL, AML patients and the normal control were 4254.37±2686.58,2399.99±904.66,2339.85±672.25 (pg/ml)respectively, the average levels in the ALL were higher than AML and normal control group(P<0.05); The average levels of SDF-1 in CNSL group and non- CNSL groups were 3700.05±438.25,3132.50±2158.58 (pg/ml) respectively, the former seemed as if higher than the latter, but without statistical significance; The average plasma level of SDF-1 in the M4+M5,Ml+M2,M3 of AML patients were 3596.55±750.44,2285.05±783.07,1833.52±592.41(pg/ml)respectively, the average levels in the M4+M5 were higher than M1+M2 and M3 group (P<0.01), the M1+M2 and M3 were lower than normal control group, but without statistical significance.2. The average CSF level of SDF-1 in the ALL, AML patients and the normal control were 4577.59±1546.35,3100.32±1405.87,1978.53±857.99 (pg/ml) respectively, the average levels in the ALL and AML were higher than normal control group(P<0.05), and ALL groups higher than AML group (P<0.05); The average CSF levels of SDF-1 in CNSL group and non- CNSL group were 5236.30±750.12,3642.66±1634.53 (pg/ml) respectively, The former higher than the latter(P<0.05); The average CSF levels of SDF-1 in the M4+M5,M1+M2,M3 of AML patients were 3366.95±1424.42,3062.37±1583.48, 2921.93±472.26(pg/ml), the average levels in the M4+M5 were higher than normal control group(P<0.05)respectively, the M4+M5 were higher than M1+M2 and M3 group,but without statistical significance3. The serum level of SDF-1 in acute leukemia patients were irrelevant to CSF lever. The serum and CSF level of SDF-1 were irrelevant to old, peripheral white blood cell,creatase and bone marrow juvenile cell.Conclusion:1.The higher CSF lever of SDF-1 significantly associated with generate of CNSL, it can be utilized as an indicator of prognosis and the guidance of clinical treatment.2.The average plasma levels of SDF-1 was not related to the CNSL, which may be due to the SDF-1 local lever.