The Clinical Application Research In Preventing Abo Hdn By The Improved Coombs Test

Objective:When the pregnant women's blood group is incompatibility with the embryo's in its body, the hemolytic disease of newborn (HDN) may be happened. Once the HDN happened, the anemia,jaundice (Nuclear jaundice),Hepatosplenomegaly even edema will happened to the illed-chidren. If there is no therapy in time, the illed-children may be going to die. HDN which caused by ABO blood group incompatibility was most commonly in clinical. Therefore, during the time of pregnancy, to detect the Anti-A (B) IgG Titers of Pregnant Women, can offer the auxiliary diagnosis of HDN's occurrence and development, and can also offer the effective prophylaxis.Methods:Adopt both classic Coombs Test and Improved Coombs Test of the Anti-A(B) IgG Titers of 0 blood group Pregnant Women whose BG is different with their husbands(O/A,O/B,O/AB), through the analysis of test data and to track the Suspected cases,to discuss the application in clinical and the difference between these two metheods in preventing the occurrence of ABO HDN.1. To detect the Anti-A (B) IgG Titers of 303 Pregnant Women's serium by classical coombs test and improved coombs test and calculate the positive rates of these two methods, and then give the statistic analysis between these two methods.2. Use the statistic software to calculate the specificity and sensitivity of improved coombs test as the classical coombs test to be the standard.3. To trace and monitor the samples that the Titer is>1:64.Results:1. The positive rate:classical coombs test is 16.17%, the improved coombs test is 17.49%. There is no statistic significant of these two methods by the SPSS 13.0 software.2. As the classical coombs test to be the standard, the specificity and sensitivity of the improved coombs test is 98.96% and 99.39%.3.48 samples'Titers are≥1:64. After one month treatment,46 samples are recoverd. The other two samples be cured till delivery but the titers still>1:64(one is≥1:128, the other one is≥1:256). Conclusion:There is a good consistency of the determination of Anti-A(B) IgG Titers of Pregnant Women between the classitical and improved coombs tests, but the improved coombs test can use less time,and much more simple and convenient in its operating than the classical coobms test,so it deserved to spread in clinical.