| Background: the Dragon’s blood is Dracaena cochinchinensis(Lour.)S.C.Chen after extraction of the resin. Clinically proven to treat bedsore and skin wound healing has a good effect, but the report does not see more for the treatment of burns. This study employs the to treat burns.treatment of burns and the pharmacodynamic studies.Method:(1) The orthogonal experiment to decide the optimal method of the Dragon’s blood-cyclodextrin inclusion complex.(2) The Dragon’s blood-β-cyclodextrin inclusion complex was prepared by saturated water solution method. Drug loading,the inclusion rate, the stability,the recovery rate and the solubility were determined by ultraviolet spectrophotometry.(3) The preparation of the dragon’s blood-β-cyclodextrin inclusion complex gel, and determine its stability, including dosage, recovery rate, in vitro transdermal absorption performance, etc.(4) 50 Kunming mice were randomly divided into five groups, namely model group, positive group, the Dragon’s blood-β-cyclodextrin inclusion complex gel high, medium and low dose group,set up Ⅱdegree scald model, After building,the mice in positive group were given recombinant human epidermal growth factor gel,mice in model group were not given medicine, mice in the Dragon’s blood-β-cyclodextrin inclusion complex gel high, medium and low dose group were given the Dragon’s blood content is 5%, 2.5%, 1% gel. To observe the wound scab over, take off a scab, edema and skin infections.(5) 36 Kunming mice were randomly divided into three groups, namely blank group, medium group and gel group. The mice in blank group were not given medicine, mice in medium group were given medium and mice in cream group were given the Dragon’s blood-β-cyclodextrin inclusion complex gel.Aim the Dragon’s blood-β-cyclodextrin inclusion complex gel to mice skin without irritation.(6) 60 Kunming mice were randomly divided into five groups, namely model group, positive group, the Dragon’s blood-β-cyclodextrin inclusion complex gel high, medium and low dose group. The mice in positive group were given Jing Wan Hong,mice in model group were not given medicine, mice in the Dragon’s blood-β-cyclodextrin inclusion complex gel high, medium and low dose group were given the Dragon’s blood content is 5%, 2.5%, 1% gel. Every mice celiac injection of 0.6% acetic acid 0.01 ml/g, the body torsion records in time and body torsion reaction times within 20 minutes. Watch the Dragon’s blood-β-cyclodextrin inclusion complex gel have analgesic action.(7) ICR mice were randomly divided into 5 groups, each group of 10, namely blank group, positive group, the Dragon’s blood-β-cyclodextrin inclusion complex gel high, medium and low dose group. The mice in blank group after double foot onto blank gel, double foot after Beijing Wan Gong daub ointment in positive mice 0.1g, mice in the Dragon’s blood-β-cyclodextrin inclusion complex gel high, medium and low dose group were given the Dragon’s blood content is 5%, 2.5%, 1% gel. With your index finger rub foot every 10 seconds after mice, The groups of mice for 2 times in a row, interval of 1 h, in the last 30 min,90 min,150minafter the treatment, each measuring a pain threshold.(8) 60 Kunming mice were randomly divided into five groups, namely positive group, model group, the Dragon’s blood-β-cyclodextrin inclusion complex gel high, medium and low dose group. The mice in positive group were given Jing Wan Hong,mice in model group were not given medicine, mice in the Dragon’s blood-β-cyclodextrin inclusion complex gel high, medium and low dose group were given the Dragon’s blood content is 5%, 2.5%, 1% gel. In the right ear before and after the two sides with 100% xylene 0.02 ml/only. Twenty minutes later, cervical dislocation executed mice, along the two ear auricle cut, with 7mm punch in auricle around an ear piece on the corresponding parts, using electronic weighing scales, with left and right ear piece weight as swelling degree, the difference between the observed the Dragon’s blood-β-cyclodextrin inclusion complex gel have anti-inflammatory effects.(9) By disc diffusion method to determine the Dragon’s blood-β-cyclodextrin inclusion complex gel antibacterial activity.Result:(1) Through orthogonal experiment, the Dragon’s blood-β-cyclodextrin inclusion complex the best preparation conditions: the Dragon’s blood and cyclodextrin ratio of 1:3, the quality of the inclusion temperature is 80℃, stirring for 2 hours.(2) The Dragon’s blood-β-cyclodextrin inclusion complex content of dispersion was(9.13±0.39)%,RSD was 4.32%(n=6). inclusion rate was(23.25±0.64)%,RSD was 2.74%(n=6). Avoid light stand for eight hours at room temperature stability is better, Low, medium and high concentration of the sample average recovery was 96.75%, 103.41% and 99.41%, RSD were 2.25%, 1.18% and 0.83%(n = 5).(3) The prepared the Dragon’s blood-β-cyclodextrin inclusion complex gel was red, content of dispersion was(5.02±0.028)%,RSD was 0.55%(n=6). Low, medium and high concentration of the sample average recovery was 98.79%,101.21% and 100.46%,RSD were 3.59%,1.83% and 1.08%(n=5). In the cold,hot and the condition of centrifugal has better stability, Absorbed in vitro penetration was better than the Dragon’s blood gel effect.(4) In burns experiment, the Dragon’s blood-β-cyclodextrin inclusion complex gel high, medium and low dose group compared with control group, the difference was statistically significant.(5) Stimulating test of pathological results show that the blank group, the matrix set, gel skin of mice were normal.(6) In mice body torsion experiment, the Dragon’s blood-β-cyclodextrin inclusion complex gel high, medium and low dose group compared with control group, the difference was statistically significant.(7) In mice hot plate experiment, the Dragon’s blood-β-cyclodextrin inclusion complex gel high, medium and low dose group compared with control group, the difference was statistically significant.(8) In mice ear swelling experiment, the Dragon’s blood-β-cyclodextrin inclusion complex gel high, medium and low dose group compared with control group, the difference was statistically significant.(9) The Dragon’s blood-β-cyclodextrin inclusion complex gel had no bacteriostatic ring.Conclusion:(1) The in vitro percutaneous absorption properties of the inclusion the Dragon’s blood-β-cyclodextrin inclusion complex gel were improved obviously.(2) The Dragon’s blood-β-cyclodextrin inclusion complex gel was promote skin healing efficacy to scald in mice.(3) The Dragon’s blood-β-cyclodextrin inclusion complex gel did not stimulate the mice skin.(4) The Dragon’s blood-β-cyclodextrin inclusion complex gel can reduce the body torsion times caused by acetic acid, twisting the body to lengthen the incubation period, have analgesic effect.(5) The Dragon’s blood-β-cyclodextrin inclusion complex gel can reduce the mice pain threshold,have analgesic effect.(6) The Dragon’s blood-β-cyclodextrin inclusion complex gel can inhibit the mouse ear swelling caused by xylene, have anti-inflammatory effects.(7) The Dragon’s blood-β-cyclodextrin inclusion complex gel had no bacteriostasis. |
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