Studise On Protective Effect Of Dragon’s Blood On Mice Injuried By X-ray

Purpose: Radiotherapy is one of the main treatments methods for malignant tumors. Radiation can kill tumor cells, at the same time, the body’s normal cells, tissues and organs also suffer varying degrees of damage from it.,which not only affect the effect of radiotherapy, but also bring great pain to patients. So how to select low toxicity, safe and effective radiation protection drugs to reduce the toxicity aroused by radiation therapy, and improve the treatment effect of cancer, has become a hot spot in the research of radiation protection drugs. Traditional Chinese medicine possess the characteristic of wide source, low toxicity and less side effects.Some with the effect of heat detoxification, promoting blood circulation and removing blood stasis, buxueyyiqi,nourishing yinand promoting leukocyte have different levels of anti-radiation. Dragon’s blood(DB) is the alcohol extracts of Dracaena cochinchinensis wood rich of lipids. Studies have shown that DB has the functions of promoting blood circulation to remove blood stasis, anticoagulant, astringing sores myogenic, analgesia, anti-inflammatory, anti tumor, lowering blood glucose, and antioxidant effect. Dragon’s blood’s phenolic compounds and active ingredient,which contain resveratrol, pterostilbene, Loureirin A and Loureirin B,can scavenge free radical. Although Resina draconis has broad pharmacological effects, the anti-radiation effect of it is rarely reported. Therefore, this paper intends to study its anti-radiation effect to provide experimental basis for further development of its clinical application.Methods: The male ICR mice were randomly divided into negative control group, positive control group and DB protection group. The mice in negative control group and positive control group were given CMC-Na by intragastric administration while the mice in DB group was given DB( 100mg/Kg、200mg/Kg and 400 mg /kg) by intragastric administration. Once a day for 14 days, all groups except for the negative control group were exposed to X-ray at the 15 th day.The distance between source and target was 70 cm,and dose rate is 0.511Gy/min.Irradiation doses are 1.0Gy、2.0Gy、3.0Gy and 4.0Gy. The mice were sacrificed at 72 h after irradiation, andmeasured the WBC,bone marrow mononuclear cells, spleen index, thymus index, spleen nodules, the number of bone marrow eosinophils polychromatic erythrocyte micronucleus rate, SOD, CAT, GSH-PX activity MDA content in liver.We used excel for data entry and SPSS19.0 software for statistical analysis. The comparison among multiple groups was based on single factor analysis of variance. The comparison between the two groups using t test, with p<0.05 as the difference was statistically significant.Results: 1. Effect of DB on the frequency of micronuclei in polychromatophilic erythrocytes of bone marrow and MDA in liver Simply given 400 mg/kg DB in mice, compared with negative control group mice MDA and the frequency of micronuclei in polychromatophilic erythrocytes of bone marrow has decreased, but there was no statistically difference(p>0.05). 2. Effect of DB on WBC of mice exposing to x-ray Before exposing to 2.0Gy X-ray,mice were given low, medium and high concentrations of DB,the WBC increased significantly compared with control group and increased with the increase of drug concentration, on the number of WBC in mice at dosages of 200mg/kg and 400mg/kg was statistically different compared with the control group(p<0.05). Though 100 mg/kg of DB has a tendency to rise the number of WBC, but there was no statistically difference(P>0.05). Mice exposing different doses of X-ray, WBC were reduced, and was more obvious with the increase of irradiation dose.There was statistically difference between negative control group and positive control group(P<0.01), Preventive given 400 mg/kg DB can increase WBC,there was statistically different compared with the positive control group(p<0.05 或 p<0.01) 3. Effect of DB on spleen index、thymus index and spleen nodules of mice exposing to x-ray Before exposing to 2.0Gy X-ray,mice were given low, medium and high concentrations of DB, can significantly rise spleen index and thymus index. And the protection effect increased as the DB dose enhanced. The spleen index and thymus index were elevated obviously after mice were given DB at dosages of 200mg/kg and 400mg/kg(p<0.05 或 p<0.01);100 mg/kg of DB has a tendency to risethe spleen index and thymus index,but it’s no statistically significant difference compared with control group(p>0.05). Before exposing to 2.0Gy X-ray,mice were given low, medium and high concentrations of DB, spleen nodules has a tendency to rise compared with control group,but there is no statistically significance(p>0.05). When the mice exposing to different dose of X-ray, the spleen index and thymus index decreased accordingly,It’s statistically significance compared with negative control group(p<0.01). The spleen index and thymus index were raised significantly after the mice were given 400mg/kg DB,there has a statistically significant difference(p<0.05 或 p<0.01). 4. Effect of DB on Bone marrow nucleated cells of mice exposing to x-ray Before exposing to 2.0Gy X-ray, mice were given different concentrations of DB,can increase Bone marrow nucleated cells. There has a tendency to rise the bone marrow nucleated cells after mice were given DB at dosages of 100mg/kg and 200mg/kg comparing to control group,but it’s no statistically significance(p>0.05). At dosage of 400mg/kg DB was statistically different compared with the control group(p<0.05). Mice exposing different doses of X-ray, bone marrow nucleated cells are reduced, and are more obvious with the increase of irradiation dose. There was statistically difference between negative control group and positive control group(P<0.01).Preventive given 400mg/kg DB can increase Bone marrow nucleated cells,there was statistically different compared with the positive control group(p<0.05). 5. Effect of DB on SOD、CAT、GSH-PX activity and the content of MDA in liver of mice exposing to x-rays Before exposing to 2.0Gy X-ray, mice were given different concentrations of DB,compared with control group. Low concentration of DB has a tendency to rise SOD, CAT and GSH-PX activity.Low and medium concentration of DB can decrease the content of MDA, but it’s no statistically significance(p>0.05). Medium and high concentrations of DB can increase the activity of SOD、CAT、GSH-PX. The differences were statistically significant compared with control subjects(p<0.05 或 p<0.01); high concentration of DB can reduce the content of MDA and the difference was statistically significance compared with control group(p<0.01). Mice exposing todifferent doses of X-ray, the activity of SOD, CAT and GSH-PX were reduced,increased MDA content,and were more obvious with the increase of irradiation dose. There was statistically difference between negative control group and positive control group(P<0.01).Preventive given 400 mg/kg DB can increase the activity of SOD, CAT and gsh-px,and decrease MDA content,there was statistically different compared with the positive control group(p<0.05 或 P<0.01) 6. Effect of DB on the frequency of micronuclei in polychromatophilic erythrocytes of bone marrow of mice exposing to x-rays Before exposing to 2.0Gy X-ray, mice were given different concentrations of DB.It can decrease the frequency of micronuclei in polychromatophilic erythrocytes of bone marrow.Low and medium concentration of DB can decrease the frequency of micronuclei in polychromatophilic erythrocytes of bone marrow,but it’s no statistically significance(p>0.05). The high concentration of DB can significantly reduce the micronucleus rate of bone marrow, the difference was statistically significant compared with the control group(p<0.01).The micronucleus rate of bone marrow increased compared with the negative control group after mice received different doses of X-ray(p<0.01). Preventive given 400 mg/kg DB can obviously reduce the irradiated mouse bone marrow polychromatic erythrocyte micronucleus rate compared with the positive control group(p<0.01).Conclusion: 1. The experimental results show that DB can obviously increase WBC, bone marrow nucleated cells in mice, spleen and thymus index caused by X-rays. 2. DB can enhancethe activity of CAT, GSH-PX, SOD, and decrease the content of MDA. 3. DB can reduce the micronucleus rate of bone marrow. DB has a protective effect on mice injuried by irradiation, the mechanism may be related to increase the activity of anti-oxidant enzyme and rise the removal of free radicals. Whether there are other protective mechanisms is needed researched.