Methodological Study Of Arterial Thrombosis Model Induced By Ferric Chloride In Rabbit

Objective: The purpose of this study was to explore a new method of inducing rabbit arterial thrombosis model by external application of 10% ferric chloride solution and transient bulldog clamp occlusion. Methods: The bilateral femoral arteries of 21 New Zealand white rabbits were randomly divided into 6 groups: group A to F. Group A to D received external application of 10% ferric chloride solution for 20 min and transient bulldog clamp occlusion for 10, 7, 5 and 3 min respectively. As control groups, group E received external application of 10% ferric chloride solution for 20 min and group F received transient artery-occlusion for 10 min only. Femoral artery blood flow was monitored continuously by Doppler flow-meter till one hour after the experiment finished. A successful rabbit thrombosis model was defined as the occurrence of complete thrombotic occlusion. Results: The rate of successful model was 100% for group A and B, 57% for group C, and 29% for group D, the type of thrombus resulted from our study is a rich- platelet mixed thrombus, however, no occlusive thrombus was observed in control groups. Conclusions: External application of 10% ferric chloride solution for 20 min plus the bulldog clamp occlusion for 7 or 10 min is a practical method of inducing arterial thrombus model in rabbit.