The Clinical Significances Of The Quantative Level Of GM-CSF And G-CSF In The Plasma Of Non-Hodgkin's Lymphoma And Its Relationship With Syndromes Classification Of Chinese Traditional Medicine

Objective:To detect the quantative level of GM-CSF and G-CSF in plasma of NHL patients before and after two cycles of chemotherapy by immunoadsoption assays, and to investigate the clinical significances of the results and its relationship with syndrome classification of TCM, thus to provide evidences for early diagnosis and therapy guidance in NHL.Methods:29 samples were collected of first-diagnosis NHL patients in Fujian Provincial Hospital from June 2008 to January 2010.In the meantime,20 healthy persons were set as control group.Furthermore,the analysis was done about the GM-CSF and G-CSF quantative level among the groups of before the cycle,after the cycle,and control group. Correlation analysis was held between first-diagnosis group and control group, and between two cycles of treatment group and first-diagnosis group. Curative effect analysis was done after two cycles of chemotherapy. Defined complete remission (CR) and partial remission (PR) as effective group, disease stable and disease progression as unresponsive group. Correlation analysis was also held between these two groups before and after two cycles of treatment. Syndromes classification of TCM was used to define the 29 patients, who were divided into the positive group(han Tan condensation+qi sputum knot) and the virtual reality table group (yin phlegm and blood stasis+yin and yang Ju virtual), and correlation analysis was held after two cycles of chemotherapy.Results:The quantative level of GM-CSF and G-CSF in plasma of first-diagnosis NHL patients was significantly higher than control group (P<0.01), and the level was significantly lowered after two cycles of chemotherapy (P<0.05). In the effective group, the down-regulation of expression level has statistical significance (P<0.05) while the unresponsive group has no significant change (P>0.05). Positive group has better curative effect, while the effect in the virtual reality table group was much worse. The expression of GM-CSF showed no difference between these two groups (P>0.05), but the expression of G-CSF was much higher in the positive group (P<0.01), which suggested that the expression of G-CSF may provide evidence in clinicalConclusion:The level of GM-CSF and G-CSF were both high in first-diagnosis NHL patients, and were both significantly lowered after two cycles of chemotherapy. This suggested that these two might be new tumor markers of reference. The positive group in TCM has better curative effect, while the virtual reality table group does worse. The relationship between GM-CSF level and syndromes classification of TCM was unclear, but G-CSF might be correlated to the syndromes classification.