The Effect And Mechanism Of Human Umbilical Vein Endothelial Cell Permeability Changes Which Induced By The Serum Of Patients With Severe Acute Pancreatitis Who Had Continuous Renal Replacement Therapy

Objective: Discuss the effect and mechanism on the endothelial permeability in patients with severe acute pancreatitis (SAP) who had continuous renal replacement therapy (CRRT) .Methods: Use patients serum before treatment,treatment 6h,treatment 20h to stimulate endothelial cells 5h,at the same time to 300mmol/L H2O2 stimulated cells as positive control,to healthy controls serum stimulate the cells as a negative control.By immunohistochemical staining,fluorescence and confocal microscopy sub-photometer detection of endothelial cell permeability examination,endothelial cell tight junctions Claudin1,Occludin and VE-cadherin adhesion junction protein expression and localization changes.Results: SAP serum before the treatment and H2O2 were the same as can significantly induce human umbilical vein endothelial cell monolayer permeability increase,CRRT after treatment,the serum-induced endothelial cell monolayer permeability decreased.CRRT treatment group significantly endothelial cell loss,and significantly increased endothelial cell space,but with CRRT treatment time,SAP serum-induced reduction of endothelial cell loss,endothelial cell gap becomes smaller,cell junction protein expression increased.Conclusion: SAP serum can increase endothelial cell monolayer permeability.CRRT treatment reduces endothelial cell monolayer permeability,increased intercellular junction protein,thereby reducing the permeability of endothelial cells.