| Objective: To investigate the resistance of frequent-used antimicrobials on clinical staphylococcous aureus from Jun 2004 to Jun 2006 in a teaching hospital of chongqing, which will be helpful for the rational administration on antimicrobials; Meantime, to study on the epidemiology of the same strains carrying disinfectant-resistance gene of qacA , which will be useful for monitoring of the resistance trend,analysing the molecular resistant-mechanism and rational administration on disinfectant & septics.Methods: Two-fold dilution agarose assay was used to measure the minimum inhibitory concentrations on Staphylococcus aurues of the antimicrobials to be tested and the representative monovalent or bivalent cationic- disinfectants; PCR was employed to detect the mecA gene and the qacA gene in the isolates, followed by PFGE analyses on the homology of these qacA-carried strains. Results: Sixty nine MRSA (56.8%) were found in 123 strains of staphylococci with the drug resistance of 92.2%, 85.7%, 87%, 92.9%, 90.9%, 100%, 100%, and 40.6% on levofloxacin, amicacin, cefazolin, clindamycin, tetracycline, erythromycin and rifampicin, respectively. No resistance were detected of these strains on vancomycin, teicoplanin,linezolid. While 12 qacA-positive strains were detected from these strains, which were identified as 9 PFGE patterns. The main pattern was A (4/9).Conclusion: The isolated rate of MRSA were high,and all of which were multi-drug resistant in this teaching hospital albeit no vancomycin-, teicoplanin-, linezolid-resistance strains were found yet. Whereas the isolated rate of qacA- gene-carried staphylococci were low, indicating no prevalence in this teaching hosipital . |
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