| ObjectiveIn this study, male mice by intraperitoneal injection of sco polamine, sodium nitrite and ethanol-induced memory impairment in miceObservation of Modified Ditan Decoction on mice behavior and memory impairment, brain chemicals and nerve cells in the system of.Modified Decoction of memory impairment in mice Ditan mechanism for the treatment of memory disorders soup flavored Ditan prov ide a scientific basis.Methods96 male Kunming mice were randomly divided into four groups, namely, the normal group, model group, Ditan soup group, flavor ed Ditan soup group, n=24. Respectively saline, saline, Ditan soup, flavored soup Ditan gavage, daily one time, a total of 21 days。Four groups of mice were trained and tested with the platfor m method of learning and memory ability, with flow cytometry de tection of brain cell apoptosis rate in mice, using immunohisto chemistry to detect AchE expression, high-performance liquid ch romatography determination of brain dopamine content in. Results1. Jumping experimental results show that:1.1 access to learning and memory impairment: Model group in the training and testing process, the number of errors increased significantly as compared with the normal grou p, the incubation period was significantly shorter than the nor mal group, and the significant difference,Shows that modeling of learning and memory barriers to be succe ssful。Modified Ditan Decoction group training results and test scores were significantly better than the model group, compared with the normal group, flavored soup group training Ditan number of errors, latency differ significantly (P<0.01), test results an d the error The no significant difference,Description Modified Ditan Decoction on barriers to learning an d memory in mice model is a significant improvement.1.2 pairs of learning and memory consolidation impairment Training period in normal group and model group, the number of errors there is no difference (P< 0.05), while the test scores the number of errors in model group than in the normal group te sting, training, a marked increase in the number of errors in m odel group, indicating a successful modeling.Modified Ditan Decoction training the number of errors was less than the training model group (P<0.05), training of the norma 1 group (P<0.05), may be flavored Ditan Decoction memory defici ts in mice have a preventive role. Test scores in model group a nd normal group, significant differences between Ditan flavored soup of tests was better than the model group, indicating a su ccessful modeling, plus or minus Ditan Decoction on sodium nitr ite caused by barriers to inhibit memory consolidation.1.3 pairs of learning and memory impairment reproduce When not modeling, training, performance and normal group and m odel group, there is no significant difference, while the flavo red Ditan Decoction group and normal group (P<0.05), model gro up (P<0.01) significant differences in learning may be flavore d soup Ditan memory deficits have some preventive effect. Test scores than the normal group, model group, latency time, t he number of errors than the normal group and the training mode 1 group, indicating modeling is successful. The Modified Ditan Tang test scores better than the normal group and model group, indicating flavored Ditan decoction on learning and memory has improved to some extent the role of representation2. Flow cytometry to detect apoptosis rate of neurons in the bra in in mice experiments showed that:Model group, positive rate of increase than the normal group sh ows that modeling is successful, Ditan Ditan soup flavored soup groups, and the apoptosis positive rate of decline in the rate of apoptosis of nerve cells shows a certain inhibitory effect, it can improve memory receded, flavored soup Ditan Ditan Decoc tion group and compared, a lower rate of neuronal apoptosis mor e, it may flavored Ditan Decoction on the role of memory impair ment becomes more apparent.3. For the impact of AchECompared with normal group, model group, the membrane and cytop lasm of neurons stained significantly better, suggesting that t he model group increased AchE expression; while Ditan decoction the membrane and cytoplasm of neurons stained lighter than the model group was significantly higher than normal group of slig htly darker color, indicating that expression of AchE Ditan Dec oction group significantly reduced. Measured by image analysis, model group, integral optical density of AchE higher than the normal group, indicating a successful modeling。Ditan Ditan Tang Jiawei Tang group and the group of integral op tical density was lower than the model, while the flavored soup Ditan Ditan Decoction group and the group than in the density there is no difference, indicating Modified Ditan Ditan soup an d soup have been improved in memory of role, but their point of view from the immunohistochemical analysis not very different.4. High-performance liquid chromatography experiments show that dopamine contentApoptotic rate in model group than the normal group, indicating a successful modeling。Ditan Decoction group and the Modified Ditan decoction decrease d apoptosis rate shows the rate of apoptosis of nerve cells hav e a certain inhibitory effect, it can improve memory deficits。Modified Ditan Decoction group and Ditan decoction ratio is eve n lower for some of neuronal apoptosis, the role of memory impa irment may be more obviousConclusion1. In male rats by intraperitoneal injection of three different animal models of drug-induced memory impairment, memory impairm ent Indeed, the damage to part of clear modeling method is simp le, the better the characteristics of simulated memory impairme nt is a mature and reliable model of memory impairment. 2. Jiawei Ditan Tang can significantly improve memory in mice wi th memory deficits in three areas:learning and memory acquisit ion, memory consolidation of reproduction of obstacles and barr iers.3. Jiawei Ditan Decoction can inhibit apoptosis of brain cells, reducing the expression of AchE cortex to enhance brain levels of dopamine in learning and memory play a role in the preventi on and improvement.4.Chinese medicine practitioners "sputum" theory and the occurr ence of memory impairment have some association Ditan Resuscita tion is to prevent and improve the memory impairment of the bas ic rule France... |
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