| Objective: Observe the effects of appetite, related to the level of leptin and Orexin secretion on the eliminating wetness-evil and soothe-liver method in treating liver-against-stomach, and FD rats, to explore the neural and biochemical basis of liver-against-stomach, and FD. Reveal the method of eliminating wetness-evil and soothe-liver in the FD treatment may affect the mechanism of appetite.Methods: A total of 64 male Wistar rats, with body weight ranging from 200 to 240 g, provide by the Experimental Animal Center of Hebei Medical University. First, rats were randomly divided into Modeling test the control group (n = 5) , Modeling test stimulation group (n = 5) and experimental group (n=54). Will be experimental group rats were randomized block design divided into normal group (A), stimulation provoked the control group (B), Chinese medicine treatment of low-dose group (C), traditional Chinese medicine treatment dose group (D), traditional Chinese medicine treatment of high-dose group (E) and western medicine treatment group (F) 6 group, n = 9 rats. Each group of rats was measured daily food intake per day, free food and drinking water, rats and food from the Experimental Animal Center of Hebei Medical University. The rats were modeling by rat tail pain stimulation methods. In addition to the normal group and model test control group, the other groups received daily stimulation model. Modeling seven consecutive days, Modeling test the control group and modeling test stimulation group after 12 hours fasting for model testing. Through the observation of rat behavior and diet, and detection of serum leptin levels in animal models verify whether the model successfully. First, from behavioral observations, stimulated rats drinking, eating and weight significantly reduced, even don't want to eat. Look at the performance of a sleepy state, and appear darker hair, brown-like changes; second, after the rats were decapitated rapidly save the blood samples, using Elisa kit for determination of serum leptin levels. Of diet, body weight and leptin levels in the data between the two sample t test. Show the difference was statistically significant, suggesting that a successful model. Cessation of the stimulus rats in each group. Normal group and stimulation provoked the control group rats given 2ml distilled water per day. Chinese medicine treatment of low-dose group given day, 0.5ml concentrated Chinese medicine Chinese medicine plus 1.5ml of distilled water gavage treatment. Traditional Chinese medicine treatment dose group given day, 1ml concentrated Chinese medicine Chinese medicine plus 1ml of distilled water gavage treatment. Traditional Chinese medicine treatment of high-dose group given a daily gavage 2ml concentrated Chinese medicine treatment. Western medicine treatment group given a daily gavage treatment Motilium 2ml solution. Concentrated Chinese Medicine eliminating wetness-evil and soothe-liver Powder stock solution for the enrichment of 1 / 10 of the concentrated solution obtained from the Institute of Traditional Chinese Medicine cooking by the Hebei Traditional Chinese Medicine Institute. After seven consecutive days, on the same day the morning was decapitated for all rats. Execution 20 minutes before each rats were gavage 1ml ink. After decapitation quickly isolated hypothalamus and save blood samples on the ice box, Using Elisa kit for determination of serum leptin levels. Using RIA kit for determination of Plasma and hypothalamus tissues of orexin A levels. Remove the entire small intestine in rats after cesarean section length measurement according to the small intestine to promote the rate of ink. According to test results of all groups observed, to explore the neural and biochemical basis of liver-against-stomach, and FD. Reveal the method of soothing the liver and regulating qi in the FD treatment may affect the mechanism of appetite.Results: 1 Appetite changes: Normal food intake remained stable. Modeling view to stimulation provoked the control group and Chinese and Western medicine treatment for all rats rapidly reduce food intake, a downward trend evident. Treatment period to stimulation provoked the control group continued to maintain a low appetite and food intake status, food intake to restore obvious. Traditional Chinese medicine treatment of low-dose group of rats to resume food intake slightly better than that stimulation provoked the control group. But the average daily food intake is still far lower than other rats. Dose of traditional Chinese medicine treatment of food intake in rats recovered to become stable. To the end of the treatment period have been restored to near normal levels of the rats daily food intake. Traditional Chinese medicine treatment of high-dose recovery in rat's food intake was evident. Treatment of late has even more than the normal food intake daily food intake in rats. Western medicine treated rats to resume food intake trends and traditional Chinese medicine treatment of high-dose group was basically the same.2 Promote the rate of the small intestine: Rats in each group promote the rate of the small intestine contrast to ink a significant difference (P <0.001). Pairwise comparison results indicate that the normal group and traditional Chinese medicine treatment of high-dose group rat small intestine between the promoting inks was no significant difference (P> 0.05). Other groups to promote the rate of ink between the rat small intestine were significantly different (P <0.05). From the mean look stimulation provoked the control group (46.44±2.74%) is slightly lower with traditional Chinese medicine treatment of low-dose group (52±3.43%). Traditional Chinese medicine treatment of low-dose group was significantly lower than that of Chinese medicine treatment dose group (63.33±3.16%). Traditional Chinese medicine treatment of high-dose group (71.44±3.09%) was higher than the previous three groups, close to the normal group (72.67±2.69%) level. Western medicine treatment group (76.44±2.51%) in the rat small intestine was significantly higher than other groups.3 Weight changes: After the initial group: body weight between the rats in each group no significant difference (P> 0.05). Modeling success: weight of rats in each group there was significant difference (P <0.01). Pairwise comparison results indicate that normal (A) body weight with the other groups were significantly different (P <0.001). In addition to the normal group body weight among the other groups there was no significant difference (P> 0.05). From the mean look to the body weight in each stimulate group significantly lower than normal group (223.33±8.82g). After treatment: body weight in each group there was significant difference in contrast (P <0.01). Pairwise comparison results indicate that traditional Chinese medicine treatment dose group (D) and Western medicine treatment group (F) between the body weights had no significant difference (P> 0.05). A group and traditional Chinese medicine treatment of high-dose group (E) compared with 0.01 0.05). The normal group (A), traditional Chinese medicine treatment dose group (D), western medicine treatment group (F) The serum leptin levels between groups was no significant difference (P> 0.05). D group, traditional Chinese medicine treatment of high-dose group (E), F group the serum leptin levels between groups was no significant difference (P> 0.05). Other groups serum leptin levels were significantly different (P <0.05). From the mean look at B group (15.73±2.48ng/ml) and the C group (14.00±1.73ng/ml) serum leptin levels were significantly higher than the other four groups. D group (11.00±2.15ng/ml), E group (10.05±1.33ng/ml) and the F group (10.93±1.61ng/ml) serum leptin levels have been close to the normal group (12.38±1.60ng / ml) levels.5 Plasma orexin-A levels in rats: The plasma orexin-A levels between the rats in each group were significantly different (P <0.001). Pairwise comparison results indicate that normal group, traditional Chinese medicine treatment dose group and western medicine treatment group of plasma orexin-A levels between groups was no significant difference (P> 0.05). Western medicine treatment group and traditional Chinese medicine treatment dose group, Chinese medicine treatment of high-dose group compared with the level of plasma orexin-A was no significant difference (P> 0.05). Other groups plasma orexin-A levels were significantly different (P <0.05). From the mean look to stimulation provoked the control group (691.67±32.05pg/ml) and Chinese medicine treatment of low-dose group (648.63±32.48pg/ml) plasma orexin-A levels were significantly higher than the other four groups. Traditional Chinese medicine treatment dose group (571.59±18.75pg/ml), traditional Chinese medicine treatment of high-dose group (552.09±19.53pg/ml), western medicine treatment group (567.19±10.61pg/ml) plasma levels of orexin-A is close to the normal group (570.14±24.05pg/ml) level.6 Hypothalamic orexin-A levels in rats: The hypothalamic orexin-A levels between the rats in each group were significantly different (P <0.001). Pairwise comparison results indicate that the normal group (A) and Chinese medical treatment in the dose group (D), traditional Chinese medicine treatment of high-dose group (E), western medicine treatment group (F) between the rat hypothalamic orexin-A level of no significant difference (P> 0.05). Stimulation provoked the control group (B) and Chinese medicine treatment of low-dose group (C) between the rat hypothalamic orexin A was no significant difference (P> 0.05). D group, E group, F group, compared between the groups in rat hypothalamus orexin A was no significant difference (P> 0.05). Other groups of rat hypothalamic orexin A levels were significantly different (P <0.05).From the mean look at B group (3.65±0.76 pg /μg) hypothalamic orexin-A level was slightly lower with the C group (3.99±0.83 pg /μg). B group, C group was significantly lower than other four groups of rats with hypothalamic orexin-A level. D group (5.25±0.64 pg /μg), E group (5.49±0.62 pg /μg), F group (5.43±0.35 pg /μg) of rat hypothalamic orexin A levels close to the normal group (5.08±0.45 pg /μg).7 The correlation between serum leptin levels and plasma orexin-A levels: Serum leptin level (x) and plasma orexin-A levels (y) showed a significant positive correlation. Linear regression equation was y=433.234+13.5240x (r=0.6342,p<0.01).8 The correlation between serum leptin levels and hypothalamic orexin-A levels: Serum leptin level (x) and hypothalamic orexin-A levels (y) showed a significant negative correlation. Linear regression equation was y=7.4652+0.2145x (r=-0.6030,p<0.01).9 The correlation between plasma orexin-A levels and hypothalamic orexin-A levels: Plasma orexin-A level (x) and hypothalamic orexin-A levels (y) showed a significant negative correlation. Linear regression equation was y=11.5789+0.0113x (r=-0.6754,p<0.01).Conclusion:1 The method of eliminating wetness-evil and soothe-liver can be markedly improved through the FD rats, food intake and body weight, the nutritional status of rats compared to non-therapeutic state of the recovery rate has accelerated noticeably.2 With the method of eliminating wetness-evil and soothe-liver as a guide. Use Chaihushugan Powder treatment FD can improve gastric motility in rats, but to improve gastric motility slightly worse than the domperidone.3 With non-treated rats compared to using eliminating wetness-evil and soothe-liver method treatment of FD serum leptin and plasma orexin-A secretion levels were significantly lower. Secretion of hypothalamic orexin-A levels was significantly increased, and as the dose increases the more obvious differences. This may be soothing the liver and regulating qi Treatment of FD role of one of the mechanisms.4 Establishment of FD model by continuous stimulation the end of rat tail. Serum leptin levels and plasma orexin-A levels showed a positive correlation, between serum leptin levels and hypothalamic orexin-A levels showed a negative correlation, between plasma orexin-A levels and hypothalamic orexin-A levels showed a negative correlation. |
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