| Objective:Construction of the test guinea pig ear model of psoriasis-like animal,through hoop around compound gall extract, positive control (0.05% Victoria A acid cream, with cream & P) and negative control agents (petrolatum ointment) for topical delivery of parallel to observe the animals in each group tested before and after treatment of ear skin changes through the skin lesions of psoriasis severity index for (PASI) score more with Baker method and compare the histological score points to explore the topical cream compound gall hoop around the feasibility and efficacy of treatment of psoriasis.Methods:(1)40 guinea pigs sub-standard breeding cage, adaptive feeding for one weeks.(2)After one weeks of 8 randomly selected as the E group were fed control group, more than 32 will be 5% propranolol emulsion evenly applied to both sides behind the ears of guinea pig skin (1cm×1cm drug 0.3g, with a glass rod Tu Yun) thickness of 1.0mm,3 times a day for 2 weeks, resulting in psoriasis-like model.(3)After 2 weeks,the guinea pigs were randomly divided into four groups,n=8,including the A group (compound gall Guwei paste the experimental group), B group (P with ointment) for the positive medicine control group, C group (0.05% retinoic acid ointment) for the Western positive control group,D group,negative control (Vaseline).(4)Ea(?) lesions in guinea pigs after the office well marked, the corresponding topical cream. Liang Jun drug control for the four groups (1cm×1cm drug 0.2g, with a glass rod Tu Yun),2 times a day, morning and evening for three weeks.(5)In drug treatment before and after three weeks of treatment, PASI assessment conducted.(6)Medication three weeks after surgical double ear of guinea pigs in each group to take the Department of specimens, set 10% formaldehyde solution in fixed, embedded in paraffin, sliced, regular HE staining and observed under light microscope each of the local pathological changes in ear skin, the light of Baker Act is organized Integral assessment of learning.(7)SPSS13.0 for Windows software with all the data for statistical analysis,all data are X±S,said T tests were used and ANOVA analysis,the comparison between groups was statistically significant difference in the further use q pairwise comparison test todo.With P<0.05 significant difference.Results: 1,Two weeks after modeling, model animal ears fall off the back applicator Department hairless part of the root hair, part of the animal behind the ear of local skin redness,dilated capillaries,and the emergence of the phenomenon of scratching the ear, the normal control group guinea pigs ears no hair off.2,After modeling, PASI score before and after treatment in each group were A group 6.75±1.04,B group 6.75±0.71,C group 6.63±0.74, D group 7±1.07,after treatment A group 2.38±0.52,B group3±0.54, C group 2.13±0.84, D group 6.25±1.29,Compound gall hoop around the experimental group and Vaseline ointment group, the difference was significant (P<0.01),cream compound gall hoop around the experimental group compared with 0.05% retinoic acid ointment,the difference was not statistically significant (P> 0.05),compound gall hoop around the experimental group and the S & P with cream cream group, the difference was significant (P<0.05).3,After modeling, the pathological guinea pig ear skin epidermis shows hyperkeratosis,parakeratosis.Acanthosis dermal telangiectasia. A more visible mononuclear cells and polymorphonuclear leukocyte infiltration. Baker score was 6.56±1.81,topica drugs in each group after the modeling office histopathological lesion; improved, Baker score decreased from Group A 2.75±0.55,B Section 3.41±0.68,C Section 2.31±0.60,in each group pairwise comparison, cream compound gall hoop around the experimental group compared with the contro group, the difference was significant (P<0.01),cream compound gall hoof around the experimental group with the S & P with ointment and comparison the difference was significant (P<0.05).Gypsum compound gall hoop arounc the experimental group and the RA group (P<0.05),the difference was no statistically significant.4,After modeling the ear skin tissue of guinea pigs showed obvious inflammatory cell infiltration in their score of 86.38±5.94,the number of inflammatory cells after treatment shows that there are differen degrees of reduction, A group of 52.37±3.56,B group 61.44±5.78,C group 45.36±5.21,the drug group compared with the vaseline group was significantly different (P<0.05).Paste Compound gall hoop around the experimental group found no local side effects caused by applied externally.Conclusion:The experimental results show that:1,Compound gall ointment foi external use can improve the hoop around the ear of guinea pigs PASI score of psoriasis-like skin.2,Ointment Compound gall Guwei propranolol-inducec epithelial proliferation in guinea pigs, the phenomenon of capillary dilation anc inhibit inflammation.3,Compound gall hoop around on psoriasis cream is effective for period, suggesting the role of its target is the dermal papilla layer after anti-angiogenesis. |
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