Clinical Observation On Psoriasis Vulgaris Treated By Chinese Herbal Compound And Metabolics Studies Of Treatment Effects On Tcells

Background:Psoriasis is a relatively common clinical chronic intractable skin diseases, because of their physical and psychological impact on the patient is multifaceted, and easy to relapse after treatment, it is difficult to cure, so the lead investigator of the relevant disciplines worldwide attention and thinking, and has carried out research in all aspects. In recent years, an increasing number of studies have shown that psoriasis is a systemic disease, with the overall sense of thinking of traditional Chinese medicine in the treatment effect has certain advantages, and actively explore new treatments, research and development of new drugs for silver disease treatment crumbs worked out a new path.Clinical observation sectionObjectiveThe clinical efficacy of traditional Chinese medicine for the treatment of psoriasis vulgaris （blood heat syndrome） was observed.MethodsIncluded40cases of psoriasis patients were randomly divided into two groups according by us, as the treatment group （traditional Chinese medicine psoriasis on the1st side group） and control group Tripterygium group. Treatment period of1month after treatment, both groups of patients, respectively, were evaluated by PASI score.ResultsAccording PASI score, cure rate of25%, the total effective rate of66.7%, control group was16.7%, with a total effective rate of58.3%. Statistical analysis comparing the efficacy between the two groups, P>0.05, not statistically significant. The two groups were not found adverse reactions.ConelusionChinese herbal compound and Tripterygium efficacy results were similar treatment of psoriasis; there is no significant difference between the treatment group and the control group, and drug safety.Metabolomic research sectionObjectiveTry metabolomics perspective to explore traditional Chinese medicine psoriasis on the1st of the mechanism of action, the use of one-dimensional nuclear magnetic resonance NMR （’H-NMR） techniques respectively containing serum in vitro cultured human intervention after the T lymphocyte metabolism in the cytosol differences of products carriedMethodsFrom healthy volunteers donated T lymphocytes isolated lymphocyte activity, combined with flow cytometry applications, separation and detection of T lymphocytes and CD3+CD8-IFN_γ+（Th1）/CD3CD8-IL₄+（Th2） cells. Into traditional Chinese medicine group, triptolide group and the saline control group, the use of flow cytometry Thl and Th2ratio, to observe the effect of serum containing traditional Chinese medicine on human T lymphocyte differentiation. Analyzed using NMR techniques （1H-NMR） T lymphocyte lysis supernatant fluid and metabolic spectrum by Simca-P software, the main component analysis, studies were serum metabolites between drugs and the impact of saline serum T lymphocytes differences.ResultsAfter the cell culture by flow cytometry showed that:the saline group, traditional Chinese medicine group, triptolide group can affect the T lymphocytes to Thl cell differentiation, but the trend is more obvious effects of Tripterygium group. Traditional Chinese medicine group, there are differences tripterygium group and saline group, while no significant difference between the group and the traditional Chinese medicine Tripterygium groupMRI test results showed that:compared with the saline group, psoriasis the1st group of L-carnitine, cholesterol, deoxyinosine, deoxyuridine iodinated tyrosine3-hydroxybutyric acid, aldosterone decreased, The p-hydroxy acid, adenine nucleosides were increased. Tripterygium fine amino acid group, p-hydroxy acid, deoxy-uridine, cholesterol, deoxyinosine, iodinated tyrosine, etc. aldosterone decreased.ConelustionAccording to the results of metabolomics studies of psoriasis patients with carbohydrates, proteins, amino acids and other metabolic abnormalities, and the citric acid cycle, gluconeogenesis, glycolysis multiple metabolic pathways, amino acid metabolism, purine pyrimidine metabolism related. May also cause damage to the intestinal mucosa barrier function and liver. Chinese herbal compound demolish the1st adjustable glycolipid metabolism in patients with psoriasis, making metabolic networks show a trend towards restoration of normal state, this may be the1st party psoriasis treatment of psoriasis vulgaris mechanism.Chinese herbal compound No.1, psoriasis can affect T lymphocytes to Thl cell differentiation, and metabolic effects on human T lymphocytes Unlike saline or Hook, differences in metabolites related to the total effect; differences in metabolites may involve lipids, proteins such as a series of intracellular biochemical substances with multiple metabolic pathways related to fat metabolism, thus optimizing the metabolic network, highlighting the comprehensive multi-target effects of Chinese medicine. Chinese herbal compound can regulate cell metabolism, so this huge trend of normalization of metabolic networks, which may be the mechanism Qingreliangxue traditional Chinese medicine treatment of psoriasis vulgaris...