The Expression And Significance Of Angiopoietin-1 And Angiopoietin-like Protein 2 MRNA In The Kidney Of Diabetic Rats

Objective:To explore the expression and significance of angiopoietin-1 and angiopoietin-like protein 2 in the kidney of the induced type 2 diabetic rats.Methods:Eight weeks old Wistar rats (n=40) were divided into two groups: control (n=15, group A) and model groups (n=25, group B). The model group rats were intraperitoneally injected low-dose streptozotocin (STZ,30mg/kg) after having the sucrose-and fat-enriched diets for eight weeks. The control group rats were given common diet. Blood glucose, HbAl c,cholesterol, triglyceride, Scr, BUN,insulin and 24-hour urinary protein excretion were detected at 8,9,12 and 16 weeks respectively.Six rats were sacrificed at 9 weeks in every group.The others were sacrificed at 16 weeks.Kidney index of rats were measured. HE staining was used to observe the renal pathological changes. The expression of Ang-1 mRNA and ANGPTL2 mRNA in the renal cortex were detected by semi-quantitative RT-PCR.Results:Some pathological characteristics of diabetic nephropathy exist in type 2 diabetic rats.Blood glucose, HbAlc,cholesterol, triglyceride,Scr, BUN,insulin,24 - hour urinary protein, kidney index were significantly increased in model groups as compared with control groups (P<0.05,P<0.01). Compared with the controls, semi-quantitative RT-PCR showed that Ang-1 mRNA and ANGPTL2 mRNA were significantly increased in the diabetic groups (P<0.01,P<0.05)Conclusions:Ang-1 and ANGPTL2 are endothelial cell growth regulating factor that correlated with vascular angiogenesis. Ang-1 and ANGPTL2 may play an important role in the pathogenesis of the diabetic microangiopathy.