The Effect Of Hydroxyethyl Starch On Brain Capillary Permeability In Rats With Severe Acute Pancreatitis

Objective To investigate the effect of hydroxyethyl starch on brain capillary permeability in rats with severe acute pancreatitis.Methods 50 male Sprague-Dawley rats were randomly divided into sham operation(SO) group, severe acute pancreatitis(SAP)group, hydroxyethyl starch(HES) treated group.Each group was divided into two subgroups as 6h and 24h,with 8 rats each subgroup except for 10 rates in 24h SAP group.Model was induced by injecting 5% sodium taurocholate into pancreaticobiliary.Serum TNF-α,the brain water content,pathological changes of brain and pancreas were measured and the expression of AQP4 was determined by RT-PCR plus immunohistochemistry 6h and 24h after induction.Result The levels of serum TNF-αin sham operation(SO) group,SAP group, hydroxyethyl starch(HES) treated group were (35.84±11.91)pg/ml,(97.14±6.70)pg/ml,(78.29±4.77)pg/ml in 6h,they were (38.58±12.22)pg/ml,(148.49±24.77 ) pg/ml,( 112.50±7.11 ) pg/ml in 24h,with significant difference(P<0.05). The water content were (76.98±0.57)%,(79.72±0.62)%,(78.13±0.25)% in 6h,they were (77.30±0.83)%,(80.44±0.54)%,(78.59±0.42)% in 24h, with significant difference(P<0.05). The expression of AQP4 mRNA were 0.5083±0.0466,0.7735±0.0243,0.6767±0.0255 in 6h,they were 0.5480±0.0488,0.8142±0.0168,0.7181±0.0188 in 24h, with significant difference(P<0.05). The expression of AQP4 proteins were 3.25±1.04,6.88±1.89,4.63±2.07 in 6h,they were 3.50±0.93,7.50±1.60,5.63±1.69 in 24h,with significant difference(P<0.05).Both in 6h and 24h,compared with sham operation group,the levels of serum TNF-α,the water content and the expression of AQP4 mRNA and proteins in SAP group were significantly increased(P<0.05).Compared with SAP group,the levels of serum TNF-α,the water content and the expression of AQP4 mRNA and proteins decreased obviously in HES treated group(P<0.05).Conclusions HES may inhibit the inflammatory factor--TNF-α,indirectly down-regulate the expression of AQP4,which may play an important role to decrease the effect of brain capillary permeability in SAP.HES may alleviate the brain capillary permeability in SAP.