Component Analysis Of Ranunculus Japonicus Thunb By HPLC, UPLC/Q-TOF-MS

Ranunculus japonicus Thunb is a widely spreaded from northeast to southChina perennial herb in China,which belongs to family of Ranunculaceae used as herb in medicine.collected in summer or autumn used by cut off,fresh or open-air drying. perennial herb in field ,roadside,border, clivas. There are some synonyms for it such as Yuliaocao,Yajiaoban,Shanlajiao,Tigerfoot herb Qipaocai and Maoqincai. Acrid,subtle bitter.warm, poisonous.This plant has Pharmacological activity, such as antichloristic, analgesia, antibacterial and antitumor. And it is used as chinese fork medicine in the treatment of Jaundice, Tuberculosis, and Hepatitis. to try to find out the material foundation of pharmacodynamic action of Ranunculus japonicus Thunb and to progress studying pharmacologic action of Ranunculus japonicus Thunb, this topic carried out the study of analysis and identification by the combination technique of Ultra-performance liquid chromatography with quadruple-time-of-flight mass spectrometry (UPLC/Q-TOF-MS). Principal work and main result showed as follow:(1) analysis the the main components in the total glycosides of Ranunculus japonicus by HPLC-PDA,to set up a method of chromatographic process of HPLC-PDA of total glycosides of Ranunculus japonicus;(2) Rapid analysis of the main components in the total glycosides of Ranunculus japonicus by UPLC/Q-TOF-MS first time,identified 20 compounds from the total glycosides of Ranunculus japonicus,most of them are flavanoid and lactone compond;(3) Analysis the the main components in the alkaloids extract of Ranunculus japonicus by HPLC-PDA,to set up a method of chromatographic process of HPLC-PDA of alkaloids of Ranunculus japonicus;(4) Analysis of the main components in alkaloid extract of Ranunculus japonicus by UPLC/Q-TOF-MS first time. identifed 15 compounds from Ranunculus japonicus,12 compounds of them are alkaloids .This study first time identified 20 compounds from the total glycosides of Ranunculus japonicus by UPLC/Q-TOF-MS,supply the evidence to the material foundation of pharmacodynamic action of Ranunculus japonicus;set up a analysis method for complicated components of chinese traditional medicine to the pharmaceutical workers as a reference.Aslo this work analysis and identified alkaloids of Ranunculus japonicus by UPLC/Q-TOF-MS first time. This work introduced a rapid feasible method for analyzing and identifing the minor constituent of complicatied components.