| Objective:To investigate the anti-epileptic mechanism of A1 adenosine receptor by observing the variation of protein in cerebral cortex during pentylenetetrazol(PTZ)-induced kindling in A1 adenosine receptor knockout mice.Methods:Firstly, identify the A1 adenosine receptor knockout mice and the wild type mice by PCR. The groups of kindle were given intraperitoneal injection with PTZ (30mg/kg). Otherwise, the control groups were given intraperitoneal injection with identical deal of normal saline. They were killed and decapitated at specific time, the protein of cerebral cortex were extracted. Using two-dimensional electrophoresis(2-DE) observe the variation of protein in cerebral cortex of mice; scan these pictures by 2-DE scanning system, compare these pictures between the A1 adenosine receptor knockout mice and the wild type mice in the kindle groups by Image Master 2Dplatinum5.0. The difference more than twice was meaningful points. The part of these disparate points was to be take mass chromatographic analysis.Results:1. Compared with the control groups, the variation of many kinds of protein in cerebral cortex was significant difference at 24h and 1month after the mice were induced in the kindle groups.2. Between the A1 adenosine receptor knockout mice and the wild type mice,in the control groups, the difference of protein in cerebral cortex was no statistically significant; however, in the kindle groups, the variation of many kinds of protein in cerebral cortex was significant difference;3. In the kindle groups, compared the A1 adenosine receptor knockout mice with the wild type mice, the proteins of down regulation were Glutaminesynthetase, Aklolase A and Glycine receptor; the proteins of up-regulation were Protein kinase C and Glyceraldehyde-3-phosphate dehydrogenase.Conclusion:The A1 adenosine receptor may be can make protein of cerebral cortex change,this may be its anti-epileptic mechanism. |
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