The Comparative Study Of Two Deproteinized Methods On Xenogeneic Bone Graft Substitute Material

ObjectTo compare the pepsin and hydrogen peroxide's deproteinization effects on bovine bone, and compare bio-mechanical properties of the deproteinized bovine bone.MethodThe pepsin and hydrogen peroxide were used to remove the protein of bovine cancellous bone. By high performance liquid chromatography mass spectrometry technology (HPLC-MS), the content of trptophan and hydroxyproline contained in the bovine bone which made by different deproteinization methods can be measured. The biomechanical testing was also done on the bovine bone. The two deproteinization methods metioned above were evaluated by the one-way Analysis Of Variance (One-way ANOVA). Besides, the fresh bovine cancellous bone and the degreased and deproteinized bones were observed by the general histological section, HE stain and optical microscope inspect.ResultsCompared to the hydrogen peroxide, the content of hydroxyproline was higher in the bovine bone which is deproteinized by the pepsin method (F=18.785, P<0.01); the content of tryptophan was no significant difference in the bovine bone no matter which the deproteinized method was used (F=1.039, P=0.393); the two deproteinized methods did not reduce the biomechanical properties of bovine bone. Tissue sections showed the bovine cancellous bones which were deproteinized by two methods mentioned above showed that there was no obvious cell remnant in the bones lacuna.ConclusionCompared to the hydrogen peroxide deproteinization method, the pepsin deproteinization method has even less destructive to the collagen type I in the bovine bone; the two deproteinization methods mentioned above have equal effects to remove the hybrid proteins in the bovine bone, and will not reduce bovine bone's biomechanical properties.