Comparing Research Of Baizhufuling Tang, Xianglian Wan And The Combination Regulating CD Rats' Cytokine And Intestinal Mucosa Repair

Objective:Researching the mechanism of three prescriptions effect the rats cytokine immune function and intestinal mucosa repair. Studying the the mechanism of curing CD by TCM prescription and providing new methods and clues.Methods:①Using Trinitrobenzene sulfonic acid and ethyl alcohol to build the rats model, using Baizhu:Fuling(1:1,1:2,2:1), Xianglian Wan and Baizhufuling Tang(1:1)-Xianglian Wan as treatment medication.Observing the pathology change based on CD rats CMDI; Using ELISA kit to determine the TNF-α, IL-1β, IL-4, IL-10 in rats blood serum and content of Gin in intestinal mucosa.Result:①Comparing to the blank group:Model group's TNF-α,IL-1βlevel distinctly ascend(p<0.05), IL-4, IL-10 level are no significant difference, Gin level distinctly ascend (p<0.05), CMDI has significant difference (p<0.05).②Comparing to the model group:Baizhufuling Tang 1:1 group, Baizhufuling Tang 2:1 group, Xianglian Wan group, TNF-a level distinctly descend(p<0.05); Baizhufuling Tang 1:2 group, Baizhufuling Tang 2:1 group, Xianglian Wan group, Baizhufuling Tang(1:l)-Xianglian Wan group, IL-1βlevel distinctly descend (p<0.05); Baizhufuling Tang 2:1 group, XiangLlian Wan group, Baizhufuling Tang(1:1)-Xianglian Wan group, IL-4 level distinctly descend(p<0.05); Each group, IL-10 level is no significant difference; Baizhufuling Tang 1:2 group, Baizhufuling Tang 2:1 group, Baizhufuling Tang 2:1 group, Baizhufuling Tang(1:1)-Xianglian Wan group, Gln level distinctly descend(p<0.05). Each group CMDI is significant difference (p<0.05).Conclusions:①The change of CMDI and CD immune target is significant differenc, conferring the CD rats'model is successes.②Baizhufuling Tang (1:1,1:2,2:1), Xianglian Wan and Baizhufuling Tang(1:1)-Xianglian Wan can down regulate TNF-αand IL-1βlevel in blood serum, Baizhufuling Tang 2:1 and Xianglian Wan down regulate entirely and obviously; Baizhufuling Tang 2:1, Xianglian Wan, Baizhufuling Tang (1:1)-Xianglian Wan can down regulate IL-4 level in blood serum, IL-10 level has no difference change,3 prescriptions effect to anti-inflammatory factors are not significant.③Baizhufuling Tang 1:2, Baizhufuling Tang (1:1,1:2,2:1), Baizhufuling Tang (1:1)-Xianglian Wan can down regulate Gln level in intestinal mucosa, Xianglian Wan can'tResearch results show:Baizhufuling Tang and combination's most of indices are better than Xianglian Wan which reflect the difference between Baizhufuling Tang and Xianglian Wan. Providing new methods for compose prescription to cure CD.