The Study Of Content Determination Method Of Oleuropein And Flavonoids In Olea Europaea L. Leaves

Oleuropein and flavonoids, which has various pharmacological activities, are the mian compounds in the leaves of Olea europaea L.(Olive leaves). And Oleuropein and flavonoids have been widely used in industries of food, medicine and cosmetics.In this paper, ethanol- water (80:20) was optimized to extract avtive ingredients from the Olea europaea L. leaves. And the test solution was further prepared by screened macroporous adsorption resin.This paper firstly confirmed the optimization detection wavelength of the AlCl3 method and NaNO2-Al(NO3)3-NaOH method. By comparing the different coloration characteristics of total flavonoids and peuropein in olea europaea L. leaves with those two coloration systems. And the AlCl3 coloration method was established to determination the content of total flavonoids and oleuropein in Olea europaea L. leaves and the extractum which was purified by HP-1 resin. Methodological evaluation of AlCl3 coloration method was performed in the detection of total flavonoids. And the results showed that this method had advantages of simplicity and convenience of operation, good stability, repeatability and precision. and low cost, and so on. This method could served as a reference for determination of total flavonoids in traditional Chinese medicine or natural product.According to the above different adsorbance properties, the indirect determining methods of oleuropein was developed by substraction of the absorbances obtained in the above two coloratioin systems. The content of oleuropein was usually determination by high performance liquid chromatography (HPLC) or gas chromatography (GC) method in previous studied. Compared with the content determined by HPLC method, the relative error of the method was< 4% with a high confidence. The result showed that this method could be used to replace the HPLC methods for the determination of the content of pleuropein in the olive leaves or its products as the former was more simple, faster and lower cost than the latter. It can be took as a reference method for the indirect determination of the active components in herbal materials of natural products. In addition, in view of multitudinous pharmacology activities of flavonoids from natural products, and the unique separation and analysis characteristics of countercurrent chromatography, the paper also reviewed the advances in application of high-speed countercrrent chromatography in separation and purification of flavonoids from natural products.