To Study Allergic Rhinitis Of TCM And IL-4 Gene Methylation Between Uighur Nationality And Han People Comparative In Yili Of Xinjiang

Objective:Allergic rhinitis syndromes in Yili area distribution and allergic rhinitis IL-4 comparison of gene methylation between Uighur nationality and Han people.Allergic rhinitis in the region get TCM distribution,whether IL-4 gene methylation have differention between Uighur nationality and Han people. Methods:Using random cluster sampling method and survey of AR patients with TCM syndrome differentiation, to obtain the prevalence of AR and the constituent ratio of TCM.To sure Uighur nationality and Han people,which have healthy control and patients. Extracted from peripheral blood, extracted DNA in the blood and DNA were modified sulfite for IL-4 MSP amplification,and analysis of the results. Results:Investigated 2413 people,including 421 patients AR,the prevalence was 17.45%. Uighur nationality prevalence was 13.76%,which was less than that in Han people (27.43%), P<0.01.It was AR in 446 patients that in line with the number of TCM 242.The highest TCM of syndrome of deficiency of lung qi(69.01%),followed by deficiency of spleen qi type(13.22%), syndrome of lung heat(9.51%), syndrome of deficiency of kidney(8.26%). The IL-4 promoter methylation was 51.61%(16/31) in Han AR,unmethylated was 16.13%(5/31),methylation and unmethy lated was 32.26%(10/31).The complete methylation was 25.81%(8/31) of healthy Han, unmethylation was 6.45%(2/31), methylation and unmethylated was 67.74%(21/31). The IL-4 promoter methylation was 48.38%(15/31) in Uigur AR, unmethylated was 25.81%(8/31),methylation and unmethylation was25.81%(8/31).The complete methylation was 19.35%(6/31) of healthy Uighur,unmethylation was 38.71%(12/31), methylation and unmethylated was 41.94% (13/31).The AR methylation compared with the control groups of Han people (P<0.05), The AR methylation compared with the control groups of Urighur nationality (P>0.05).It was no difference of AR between Han and Uighur nationality (P>0.05),There have significant differences with healthy control of methylation complete between Han people and Urighur nationality(P<0.01). Conclusion:The prevalence of AR in Uighur nationality,which was less than that in Han people, deficiency syndromes in lung type than the other three types.Climate and environment that have a certain influence on the AR.There have some influence on the incidence between Uighurs nationality and Han people living habits and environment.It is no difference between the two nations AR positive,but in Han people have a difference in IL-4 gene methylation.It was a feasible approach as the study of allergic rhinitis that IL-4 gene methylation.The methulated and unmethylated of Uighurs healthy controls which was less than Han.The IL-4 methylation of Han people might tend to expression which susceptible to allergic rhinitis.