Investigate The Levels Of CD4~+CD25~+CD127~- Regulatory T Cells In Peripheral Blood Of Patients With Cervical Carcinoma

Objective:This study aimed to observe the differences in the levels of the CD4+CD25+CD127- regulatory T cells in the patients with cervical carcinoma and learning the relationship between the infection of reproductive tract high-risk human papillomavirus (HR-HPV) infection types and cervical carcinoma. Methods:The percentages of CD4+CD25+CD127- regulatory T cells in the peripheral blood lymphocytes were determined by flow cytometry in 60 patients with cervical carcinoma and 60 cases with controls group, And the difference of HR-HPV spectrum on two groups and coincidence rate of TCT and histopathology diagnosis. Results:1 The proportion of CD4+CD25+CD127-Treg in the peripheral blood lymphocytes from patients with cervical carcinoma (9.062%±1.132%) were significantly increased compared with those from the controls group (6.037%±1.701%), P<0.01; 2 The positive rate of HR-HPV in cervical carcinoma was 93.33%(56/60), while 21.67%(13/60) in healthy people. There were significant differences of HR-HPV types infection between cervical carcinoma group and controls group (P<0.01).3 There were no deference of HR-HPV multiple infection and single variant infection between cervical carcinoma group and controls group.4 The levels of CD4+CD25+CD127 Treg in the peripheral blood of the patients with HR-HPV positive were significantly higher than the HR-HPV negative; 5 The abnormal rate of TCT in control group was 63.64%, while was 92.86% in cervical carcinoma group.Compared cervical carcinoma group with control group, they had significant difference(校正x2=6.572, P=0.010). Conclusions:1 CD4+CD25+CD127 Treg and HR-HPV types infection may play an important role in the oncogenesis and development of cervical carcinoma; HR-HPV genotypes were mainly HPV16 subtype in cervical carcinoma.2 There were no evidence that HR-HPV multiple infection could aggravate the cervical neoplasia.3 The selective rate of cervical carcinoma was increased by TCT combined with HR-HPV subtypes testing.