| To study analgesic and anti-inflammatory effect, control AA rats serum IL-1, IL-4, IL-10, RF, TNF-αfunction, lighten synovial pathological changes and long-term toxic reaction of Feng Shi Tong Xiao Pill.200 mice and 140 wistar rats are breeded normally in SPF laboratory 10 days. 50 mice external are given xylene to make swelling and distension model and other mice are taken hot plate model, radiogenic heat irradiate model and writhing model to do analgesia experiment. Every model has 50 mice and the mice have been grouped into a blank group, a low dose group, a middle dose group, a high dose group and an aspirin group, every group has 10 mice. 50 female mice are used in hot plate experiment, the rest of the and male and female mice each half at other model. 60 rats have been grouped into a blank group, a model group, a control group and a Glucosida Tripterygii TOTA group, 30 rats are shared model group and every group has 10 rats for remaining rats groups, male and female half. Every rat right back digiti pedis that in model group, control group and Glucosida Tripterygii TOTA group has been injected 0.1ml Freund's complete adjuvant. 10 rats in model group were killed at 7th and 14th after made model, ankle joint were taken and observed synovial membrane by light microscope. At 15th after made model, rats in blank group and remanent rats in model group have been given 5% carboxymethylcellulose sodium solutions, the rats in control model group have been given Feng Shi Tong Xiao Pill auspended matter (0.075g·mL-1) and the rats in Glucosida Tripterygii TOTA group have been given Glucoside Tripterygium Total auspended matter(0.005g·mL-1), 2ml for every rat and twice a day, 8:00 and 20:00.After 15 days, the rats were killed and the blood were collected, assay serum IL-1, IL-4, IL-10, RF, TNF-αfunction by ELISA, ankle joint were taken and observed synovial membrane by light microscope. 80 rats were grouped into blank group,high dose group, middle dose group and low dose group, 20 rats were shared assigned to each group. 80 rats were given different concentration Feng Shi Tong Xiao Pill auspended matter, high dose group is 8g·mL-1, middle dose group is 4 g·mL-1, low dose group is 1g·mL-1 and blank group is 5% carboxymeth-ylcellulose sodium solutions. After three months, 10 rats in every group were killed, blood was collected, then assay blood biochemistry and kidney, liver, stomach were observed pathological change light microscope. 40 rat were breeded residuary and will be made the same after 2 weeks.The middle dose Feng Shi Tong Xiao Pill control the xylene make the external ear swelling and distension, hot plate model, radiogenic heat irradiate model and writhing model has significant difference compared with blank group, low dose group and high dose group(P<0.05), and has not significant difference compared with aspirin group(P>0.05). At checking out cytokine, the IL-1, RF, TNF-αin control group rats blood serum is lower than model group(P<0.01) and IL-4, IL-10 is higher than model group, the synovial membrane pathological change has significant change compared with model group, but not significant different compared with Glucosida Tripterygii TOTA group(P>0.05). At the study on toxicity tests, the blood biochemistry of the medicine group rats has not significant difference compared with blank group(P>0.05), and the kidney liver and stomach have no significant damage.Feng Shi Tong Xiao pill has obvious anti-inflammatory and analgesic function, can control synovial membrane pathological change, can control five kinds of cytokine in AA rats serum and is low in hilgh—dose and long course of treatment. It can be administered safely. It is a better compound recipe traditional Chinese drug to control RA clinically. |
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