CD24 Polymorphisms And Genetic Susceptibility Of Chronic Hepatitis B Virus Infection

Background The aim of this study was to investigate the role of CD24 gene in susceptibility to chronic Hepatitis B Virus (HBV) infection using family-based association analysis method. The Causes of chronic hepatitis B leading to the occurrence include the exogenous complexity factors and genetic factors. Epidemiological studies have shown that the susceptibility of different individuals in the population are quite different, this difference may be mainly determined by genetic factors. It has been found that a number of gene polymorphisms human associated with susceptibility to chronic hepatitis B. we found CD24 molecules relying on various glycosylation patterns and different cell types mediated by CD24 ligand play different biological functions. New research shows that it played an important role in maintaining immune homeostasis, regulation of immune responses. Some studies suggested CD24 gene polymorphisms be susceptible to a variety of autoimmune diseases. This study was to investigate the CD24 gene polymorphisms with chronic hepatitis B Infection.Methods Polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) was used to analyze the genotype single nucleotide polymorphism nucleotide (SNP) P170 of the CD24 gene. A family based-association test was carried out to explore the association between gene polymorphism and CHB . A total of 476 patients with CHB from 230 families were recruited. In addition, 320 family members of these patients were also genotyped. The genotype was as follows: T/T, T/C and C/C. We studied the SNP in the CD24 with respect to genetic susceptibility to CHB. Results Among 476 CHB patients, the frequency of CD24 gene P170 C, T alleles was 35.5 % and 64.5%, the frequency of CD24 gene P170 C /C, C/T and T/T genotypes frequency was 9.3%, 50.4%, 10.3% respectively. In CD24 single loci analysis by FBAT, the relationship between P170 polymorphisms and chronic HBV infection using family-based association test (single-marker FBAT analysis) showed that the alleles of T and C significantly are not associated with chronic HBV infection (P>0.05) in additive model(Z=0.169, P=0.866; Z=-0.169, P=0.866, respectively), dominant model(Z=0.522, P=0.602, Z=0.428, P=0.669, respectively) and recessive model(Z=-0.428; P=0.669; Z=-0.522, P=0.602, respectively). Transmission-disequilibrium test (TDT)and sib Transmission-disequilibrium test (STDT) did not showed an excess of the allele of T or C from heterozygous parents to affected offspring or higher frequency of alleles in the patients than their normal sibilings (χ~2=0.06, P=0.897). Our study suggested that a single nucleotide polymorphism (P170) in CD24 gene may be not significantly associated with the susceptibility to HBV and related phenotypes.Conclusion A family-based association study was used to explore the relationship between CD24 gene polymorphism and chronic HBV infection about Chinese patients with CHB. Our studying suggested that the CD24 gene may be not the susceptible gene to CHB in Chinese population by FBAT.