| Objective:To investigate the correlation of the polymorphisms in TRAIL-DR4 gene (-972C/T, -397G/T) with Susceptibility, Clinicopathologic Characteristics and prognosis in patients with NSCLC.Methods:1.Resouce:Basing on information from databases of NCBI and HapMap, others'finding, we chose -972C/T, -397G/T at TRAIL-DR4 gene promoter region and did sequencing. Chose 92 people in NSCLC as case group and 92 healthy persons as control group,which accorded with researching condition.2.Empirical method: The case group collected pafaffin blocks for isolating DNA using Formalin-fixed Paraffin-embedded (FFEP) DNA Kit; the control group collect flesh whole blood for isolating DNA using blood DNA Kit. TRAIL-DR4 gene (-972C/T, - 397G/T) genotypes were determined by using Polymerase Chain Reaction -restriction fragment length polymorphisms assay (PCR-RFLP) method.3.Stastics method:Unconditional logistic regression model was used to analyze susceptibility of NSCLC, the statistical association of genotypes and clinicopathologic characteristics, adjusted by gender, age and a history of smoking. Survival curves were analyzed by Kaplan-Meier method (log -rank test ). Cox regression model analyzed the influencing factors of prognosis and stratified analyses by the connecting ones.Results:1.Analyze of susceptibility: The -972C/T alleles were not associated with lung cancer susceptibility. Researching on the association of -397G/T alleles and susceptibility of NSCLC found that the one who had smoked more than 20 years, the genotypes including alleles T were associated with a considerably increasing risk of NSCLC (P=0.019,0R= 3.462, 95%CI=1.222-9.811).2. Analyze of Clinicopathologic Characteristics: The -972C/T alleles were related with pathological differentiation of NSCLC cell. The stratified analyses by pathological differentiation found that the degree of pathological differentiation with CT genotype is much worse than those with CC or TT genotypes (P=0.012,0R=7.599, 95%CI=1.564 -36.917). But we found that the -972C/T alleles were not associated with other clinicopathologic parameters (including pathology type, clinical stage, size of tumor and lymph node involvement). The -397C/T alleles are not associated with any clinicopathologic parameters referred in the research.3. Analyze of prognosis: Both the -972C/T alleles and the -397C/T alleles were not associated with survival rate and the prognosis of patients with NSCLC. Moreover, analysis by Cox regression found that different pathological types, pathological differentiation and clinical stages influence the prognosis of patients with NSCLC.Conclusions:1. -397G/T genetic polymorphisms has some relationship with NSCLC suscep- tibility, the genotypes including alleles T can increase the risk of NSCLC.2. The -972C/T alleles are related with pathological differentiation of NSCLC cell. Patients with NSCLC who was with heterozygote of -972C/T genotypes may have an unfavorable clinical outcome.3. Different pathological types, pathological differentiation and clinical stages influence the prognosis of patients with NSCLC. So we can infer that there may have some correlation between -972C/T alleles and the prognosis of patients with lung cancer, binding with last conclusion. However, more and deeper research is needed. |
PDF Full Text Request