Effects Of Treadmill Exercise On Learning And Memory Of AD Rats And ChAT,AchE,GSK-3β And Tau MRNA In Hippocampal

Alzheimer's disease (AD) is a progressive cognitive impairment and memory-based central nervous system damage degeneration, its pathogenesis is very complex and large number of foreign study found that exercise training can promote AD animal model of pathological learning and memory improvement and improved. The large number of animal studies have proved that appropriate training can promote learning and memory, advanced features such as the improvement of the brain, while the advanced features of sport for the mechanism of the brain is biological function in brain and nerve signal transduction linked. At present, the mechanism of AD has in-depth molecular level. In this paper, Morris water maze and real-time quantitative PCR were used to test the effects ofeight weeks aerobic treadmill exercise on the D-gal AD rats in learning and memory ability and hippocampal cholinergic and Tau protein related mRNA expression level, exploringthe use and mechanism of ChAT and AchE in the process of exercise training improved the ability of learning and memory, and the impacts of exercise training on Tau protein and the relationship with AD.In our experiment,30healthy male SD rats were chosedrandomly and divided into sedentary control group(C group, n=10), exercise injection group(S group, n=10) and sedentary injection group(D group, n=10). In these groups, C group rats were feeded normally; S group rats were injected with D-galdaily for eight weeks and were gived aerobic treadmill exercise5 times every week for eight weeks; D grouprats were injected with D-gal but not training. The injection amount of D-gal were 120mg/(kg·d) in the injection groups.At the 9th week after the experiment, Morris water maze was usedtothe three groups rats for a 5-day navigation test to test the learning ability; in the 6th day, the Morris water maze was used to the three groups of rats for space exploration experiments to testthe ability of memory and distinguish; in the 7th day the three groups of rats were killed off and the hippocampal were taken from their brains. Then we used RT-PCR to test the mRNA expression levels of hippocampal ChAT, AchE, and GSK-3βand Tau protein of the three groups rats.Experiment Results:(1) Three groups of rats were testedby Morris water maze, we found that eight weeks of D-gal injection led to the S group and D group with a decline in learning and memory, but the eight weeks of aerobic treadmill exercise has reversed thedecline of learning and memory of S group. (2) Eight weeks aerobic treadmill exercise for the proposed AD D-gal expression in rat hippocampal ChATmRNA and maintain the level of expression of the hippocampalAchEmRNA,which suggested that exercise training has promoted the cholinergic function system.(3) Eight weeks aerobic treadmill exercise has inhibited GSK-3βmRNA expression levels,which is arelatedkinase of abnormal phosphorylation Tau ofthe D-gal AD model rats, andeight weeks of aerobic treadmill exercise hasalso inhibited theover-expression of Tau protein.Conclusion:(1) Eight weeks D-gal injection can lead to the decline body weight and the ability of learning and memory of normal SD rats.Showing that eight weeks D-gal injection will successfully transformatthe normal SD rat into AD model rats.(2) Eight weeks aerobic treadmill exercise improved theability of spatial learning and memory of the D-gal injection AD rats.(3) Eight weeks aerobic treadmill exercise can promote the key enzyme ChAT and AchEmRNA expression level ofcholinergic system in hippocampal of theS grouprats.Suggesting that suitable aerobic exercise can improve the cholinergic system-related enzymes gene expression levels, and promote the ability of learning and memory and control the incidence of AD.(4) Eight weeks aerobic treadmill exercise inhibited the Tau protein and the hyperphosphorylation of Tau protein associated protein kinase GSK-3P mRNA expression levelsin hippocampal of theS grouprats.Suggesting thatsuitable aerobic exercise can inhibit the overexpression of Tau protein, inhibit the abnormal phosphorylation of Tau protein-relatedprotein kinase mRNA expression level, and inhibit the occurrence and development of AD.