Protection Against Heat Stress Injury Of Deuterohemin Peptide In C.Elegans

Peroxidases are an Important class of iron Porphyrin-containing enzymes,they catalyze Substrate oxidations used hydrogen peroxide,but the Therapeutical potential of natural Peroxidases is limited by their Scarce solubility,instability,cost and other factors.In our Previous work,we designed a novel Peroxidase Mimics Deuterohaemin-Ala-His-Thr-Val-Glu-Lys(DhHP-6)and Investigated its Peroxidase Activity and Anti-cataract activity In vitro.The peroxidase Activity of DhHP-6 is very similar to that of MP-11,yet DhHP-6 has very good water Solubility,stability and its Chemical synthsis is much more Simple.This paper use C. elegans as a model organism to establish two simple models of heat stress to investigate the effect of a synthetic mimics of peroxidase ,DhHP-6 ,for the survival rate and lifespan in both heat circumstances.Experiments proved that whether at 30℃or 35℃,DhHP-6 have a protective effect on the wild type of C.elegans under the heat stress in a sir-2.1 and daf-16 independent way . Furthermore this experiment explore which pathway the heat protective effect of the DhHP-6 for C.elegans may be adopted and propose a possible mechanism :The present of DhHP-6 can decrease the high oxidation level caused by heat shock and accelerate the main response to stress by shortening the time for HSP's up-regulation.