| Objective To investigate the associations polymorphism of apoM and plasma concentration of apoM with CHD,analyse the relationship between apoM and blood lipid. Method Three groups of subjects were enrolled: group1,patients with coronary heart disease(CHD,n=112);group2, control group who are subjects with normal coronary artery on angiography (NCA,n=168). ApoM genetic polymorphism were measured using an allele gene specific polymerase chain reaction(ASPCR) technique.The concentrations of serum apoM were measured using an ELISA technique.Results (1)The genotype and allele frequencies of the C-1065A were different in both coronary heart disease and the controls.(χ~2=11.079,df=2,P=0.004). The A allele of the C-1065A SNPs in coronary heart disease patients was significantly higher than that of the healthy controls.(41.5% vs30.4%,χ~2=7.376,df=1,P=0.007).(2)The genotype and allele frequencies of the T-778C were similar in both ischemic stroke patients and the controls.(χ~2=9.548,df=2,P=0.008). The C allele of the T-778C SNPs in coronary heart disease patients was significantly higher than that of the healthy controls.(15.2%vs7.4%,χ~2=8.538,df=1,P=0.003).(3)CHD patients with CT/CC genotype had significantly higher concentrations of TC than those with TT genotypes(t'=-3.475, P=0.001).There is a similar trend in controls ( t'=-4.441, P<0.001). TG, LDL-C and HDL-C concentrations do not correlate with apoM genotypes.(4.)CHD patients with had significantly higher concentrations of apoM than those in the control .(t=7.928, P<0.01).Conclusion (1) The C allele of the T-778C SNPs is possibly associated with increased risk of CHD. The C allele of the T-778C SNPs is possibly associated with concentrations of TC.(2) The C allele of the C-1065A SNPs is possibly associated with increased risk of CHD.(3) CHD patients with had significantly higher concentrations of apoM than those normal coronary artery on angiography.(4)ApoM is possibly a predictable factor in CHD. |
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