Effect Of Nitric Oxide And Nitric Oxide Syntheses In Allergic Rhintis

ObjectiveTo study the Effect and expression of nitric oxide and nitric oxide syntheses in allergic rhintis. MethodsAn AR model was established in healthy rats by using ovalbumin, where the AR animals were intranasally challenged with SP, and the symptoms and signs were observed and compared with those of normal controls. Meanwhile, histopathologic changes of nasal mucosa were observed with HE staining, and the density of NO3-/NO2-in the serum and nasal lavage fluid was determined by nitrate reductase method to assay the content of NO. ResultsSP stimulated typical symptoms in normal group similar to those in AR group, and aggravated the symptoms and histopathologic inflammatory reactions in AR groups(P<0.01). The density of NO in the serum and nasal lavage fluid of AR groups increased significantly after challenge with SP, and NO tended to increase with the increase of stimulation time(P<0.05). ConclusionNO and NOS induce and aggravate allergic inflammation.