Expression And Correlation Of Human Lipoxin A4 And Its Receptor,Nuclear Factor-κB P65 In Preeclampsia

Objective: Preeclampsia is an exaggeration of a systemic inflammatory process.The purpose of this study was to evaluate the role of an antiinflammatory lipid mediator, lipoxin A4 (LXA4), and the Activation of transcription factor NF-κB p65 in Preeclampsia.we measured the level of LXA4 in blood serum and the level of lipoxin A4 receptor (ALX-R), NF-κB p65 mRNA,protein expression in placenta tissues obtained from the patient with preeclampsia and normal pregnancy,to explore the difference and relationship between them and provide the evidence of experiment theories for clinical treatment and scientific research.Methods: Study group:women with preeclampsia (n=30,mild=10, Severe=20).control group:normal pregnancy(n=20). Compare they are clinical feature .Levels of LXA4 in blood serum were measured by ELISA. Reverse transcription-polymerase chain reaction (RT-PCR) were performed to detect mRNA for ALX-R and NF-ΚB p65. localized protein expression using Immunohistochemical staining.Results:1.There were no statistical difference between preeclampsia and control group in materal age,Pre-pregnancy body weight and blood pressure.(p>0.05).but women with preeclampsia were higher than control group in MAP,24h urinary protein and CRP(p<0.05).2.Levels of lipoxin A4 in women with mild preeclampsia significantly high (p<0.05).there was no significantly statistical difference between normal pregnancy and severe preeclampsia (p>0.05)3.The mRNA expressions of ALX-R was significantly low in women with preeclampsia than in women control (p<0.01); contrarily, the mRNA expressions of NF-ΚBp65 was high in women with preeclampsia than in control group (p<0.05).4.Immunohistochemical staining for ALX-R protein was located at the cytoplasm,express in villous trophoblastic cells and blood vessel endothelial cell. The expression of ALX-R protein in trophocyte showed significant lower in women with preeclampsia than in control group (p<0.01).Immunohistochemical staining for NF-κBp65 protein was located at the cytoplasm and nucleus of the cytotrophoblast. express in villous trophoblastic cells and.and blood vessel endothelial cell.The expression of NF-κBp65 protein in trophocyte showed significant higher in women with preeclampsia than in control group(p<0.01).5.The expression degree of NF-κB p65 and ALX-R was negative correlate in the severe preeclampsia and control group(P<0.05).LXA4 and ALX-R decrease together in the severe preeclampsia.Conclusion:1.An excessive maternal inflammatory response in Preeclampsia,LXA4,ALX-R and NF-κB p65 parricipated the process of preeclampsia.2. Levels of lipoxin A4 was higher in mild preeclampsia may be attribute to stress. LXA4 and ALX-R underproduction with decreased expression in women with severe preeclampsia. These findings suggest that severe preeclampsia may result from a defect in LX-mediated counterregulatory signaling. Thus, this defection in severe preeclampsia would serve to perpetuate the exaggeration inflammation and aggravate disease. 3.Because of LXA4 downregulation of proinflammatory gene expression via inhibition of the NF-κB pathway. the defection of LXA4 and ALX-R in women with severe preeclampsia. would aggravate the progress of the disease.