Influence Of Oxymatrine On Cell Proliferation And Expression Of E2F1 And C-myc In Human Hepatocelluar Carcinoma Cell Line Bel-7404

Objective: To investigate the influence of oxymatrine on cell proliferation and expression of E2F1 and c-myc in human hepatocelluar carcinoma cell line Bel-7404.Methods: Human hepatocelluar carcinoma Bel-7404 cells were cultured in vitro and treated with oxymatrine and cisplatin, then Bel-7404 cell proliferation was examined by the method of MTT. Inhibition effect of cell proliferation in human hepatocelluar carcinoma cell line Bel-7404 in different dose and different time of oxymatrine and cisplatin was detected. The group of cisplatin was the positive control group. And the expression of E2F1 and c-myc in human hepatocelluar carcinoma cell line Bel-7404 treated with IC50 oxymatrine for 72h was detected by real-time PCR assay.Results: The inhibition rate of oxymatrine with the concentration of 0.5mg/ml,1.0mg/ml,2.0mg/ml,4.0mg/ml and 8.0mg/ml on human hepatocelluar carcinoma cell line Bel-7404 for 48h and 72h were 4.31%,11.31%,19.63%,39.73%,83.10% and 6.83%,16.09%,30.92%,58.72%,97.89%, respectively. The inhibition rate of cisplatin with the concentration of 0.5μg/ml,1.0μg/ml,2.0μg/ml,4.0μg/ml and 8.0μg/ml on human hepatocelluar carcinoma cell line Bel-7404 for 48h and 72h were 6.24%,17.87%,29.59%,43.94%,72.06% and 27.63%,37.81%,54.98%,63.41%,90.62%, respectively. Compared with control group,there were significant difference in inhibited effect of oxymatrine and cisplatin on the proliferation of human hepatocelluar carcinoma cell line Bel-7404 respectively (P<0.05). The inhibited effect of oxymatrine and cisplatin was dose and time dependent. Compared with negative group, the up-regulated E2F1 and down-regulated c-myc were observed in the group of IC50 oxymatrine and their ratio were 2.33 times and 0.86 times, respectively.Conclusions: The results suggest that oxymatrine would have obvious inhibition on cell proliferation in human hepatocelluar carcinoma cell line Bel-7404, and there was dose and time dependent. Its mechanism may be related to up-regulation of E2F1 and down-regulation of c-myc.