Preliminary Study On The Pharmacy And Pharmacodynamics Of Fanbaicao

Fanbaicao first contained in the Jiu huang ben cao,is the whole plant with root of Rosaceae Potentilla species. Compendium of Materia Medica Records,it has the effect of Heat, cooling blood, detoxification, bleeding and swelling. More for the treatment of Dysentery, malaria, carbuncle swollen and various bleeding.Because of its extract has specific pharmacological activity, people carried out extensive research on the chemical constituents of Potentilla discolor.The main chemical ingredients terpenoids,steroids, flavonoids and polyphenols, etc. Recent studies have reported that it has a hypoglycemic effect. The activity in more with the flavonoids.Acute toxicity test showed that mouse maximum tolerated dose of oral Discolor is 400g/kg. Equivalent to 960 times of Clinical daily dose.Almost non-toxic side effects.The application of clinical is reliable. In addition,it has the advantages of cheap, easy to capture and so on.It is a Chinese herbal medicine with Property values treatment of diabetes.The subject is the natural fund projects in Heilongjiang Province. Traditional Chinese medicine in China as the research object.Focuses on the effect of the material basis of the hypoglycemic pharmacodynamics.Optimize extraction technology of the effectiveness.And validation for pharmacodynamic. On this basis,Developed its extract capsules, Established quality standards for preparation, provides a theoretical basis and experimental data for the development and utilization of grass.Adopt UV Spectrophotometry establish the assay method of Flavonoids in Potentilla and determination of total flavonoids in different extracts. Adopt orthogonal experimental design method to optimize the extraction process of the active site. Investigated ethanol concentration, Extraction time, Solvent ratio and extraction times. Optimum extraction of Ethanol, 70% ethanol, 12 times the volume, Reflux extraction 2 times, Every 2.5 hours. Investigated the amount of water, extraction time, extraction times. Identified the best water extraction, 10 times the amount of water, 2 times, each time 1.0 hours.Induced diabetic mouse model with 95mg/kg dose of intravenous injection of alloxan. Observed Discolor water extract on blood glucose of diabetic mice and normal glucose tolerance in mice. The results compared with model control group. The high-dose group of ethanol extract has a certain hypoglycemic effect (P <0.05). And better than Xiaoke Pill group. Glibenclamide tablet group has a significant hypoglycemic and significantly different (P <0.01). Showed that ethanol extract of Potentilla discolor alloxan diabetic mouse model has hypoglycemic effect of blood glucose, which can significantly reduce glucose tolerance in normal mice. But the aqueous extract of Potentilla discolor alloxan diabetic mouse model of blood glucose has no hypoglycemic effect.Adopt HPLC established determination of quercetin in Potentilla discolor Capsules. Refer Pharmacopoeia 2005 edition, Effects the quality inspection on the capsules. Include traits, load difference ,determination of Water, disintegration, microbiological, Heavy Check and arsenic salt check. The test results are all compliance.