Study On The Role In Regulating The Lipid Of Berberine On The Dyslipidemia Of Type 2 Diabetes

Type 2 diabetes is a metabolic disease that requires an integrated treatment. Type 2 diabetes with dyslipidemia can lead to serious complications such as heart and brain, and reasonable glucose lipid-lowering in diabetes treatment plays an important role. Coptis chinensis firstly set out in the Eastern Han Chinese "Shen Nong Ben Cao Jing", it's a commonly used Chinese medicine in the treatment of diabetes. Coptis contains many alkaloids, with berberine as the main component. Through the research of different doses of berberine's effects on the blood glucose and lipid of type 2 diabetic rats and clinical observation of berberine's effect on the blood glucose and lipid in patients with type 2 diabetes, this study evaluate the role in regulating the lipid of berberine on the dyslipidemia of type 2 diabetes, and explore the lipid-lowering effect of different doses of berberine.Section one Study on the role in regulating the lipid of berberine on type 2 diabetic ratsObjective:To study the lipid-lowering effect of different doses of berberine on type 2 diabetic rats.Method:Feeding wistar rats with high energy, high fat diet for 20 days, combined with the low dose streptozotocin (STZ) intraperitoneal injection method, we established the wistar rat model of type 2 diabetes. Randomly divided into 8 groups, treated with the distilled water, low doses of berberine (75mg/kg), medium doses of berberine (150mg/kg), high doses of berberine (300mg/kg) and metformin, simvastatin, gemfibrozil respectively with the method of intragastric administration. Collected the blood respectively after 2 weeks of treatment and 4 weeks of treatment, mainly observed the changes of the contents of serum triglyceride (TG), free fatty acid (FFA), low density lipoprotein (LDL-C), high density lipoprotein (HDL-C) and total cholesterol (TC).Result:After 2 weeks of administration, the low dose berberine group show hypoglycemic effect (P<0.05), after 4 weeks of administration, the low dose berberine group (P<0.05), medium dose berberine group (P<0.01) and high dose berberine group (P<0.01) showed significant hypoglycemic effect.The difference of hypoglycemic effect between different doses of berberine group was not significant (P>0.05).Low dose berberine group showed the result of reducing TG and TC(P<0.05) after 4 weeks of administration; the medium dose berberine group did not show the result of reducing TG and TC; large dose berberine group treated for 4 weeks showed the result of reducing TG (P＜0.05).Conclusion:Different doses of berberine all could reduce the blood glucose, the lipid-lowering effects of different doses of berberine were not consistent. The hypoglycemic and lipid-lowering effect of low dose berberine group was more obvious than medium dose and high dose group.Section two The observation of lipid-lowering effect of berberine on type 2 diabetesObjective:To study the hypoglycemic and lipid-lowering effect of berberine on type 2 diabeticMethod:60 patients with type 2 diabetes and dyslipidemia were randomly divided into berberine treatment group and simvastatin positive control groups, berberine treatment group was treated with berberine (each 0.3g,3 times a day), the control group (simvastatin 10mg), treated for 4 weeks, then observed the changes of the contents of fasting plasma glucose (FPG), TC, TG, HDL-C, LDL-C, ALT, AST, BUN, Cr, and body mass index (BMI) before and after the treatments.Result:Compared the content before and after the berberine treatment, and also the simvastatin group.1. The TC content before and after berberine treatment was significantly different(P<0.01), it decreased significantly after treated with berberine. The TC content in simvastatin control group also decreased significantly (P<0.01), and the effect of simvastatin on reducing TC was better than the berberine(P<0.01).2. The effect of berberine on reducing TG was not obvious, while the simvastatin showed better effect on reducing TG(P<0.01).3.The berberine treatment group showed the effect on increasing HDL-C, and the HDL-C content before and after berberine treatment was significantly different(P<0.05),aslo was the simvastatin control group(P<0.05).And there was no significant difference between the effects of the two group on increasing HDL-C.4. The berberine treatment showed little effect of reducing LDL-C,the LDL-C content before and after berberine treatment was not significantly different(P>0.05),but the simvastatin control group showed significant effect of reducing LDL-C(P<0.05).5.There were no observed functional lesion of liver and kidney in berberine treatment groupConclusion:Clinical observation indicated that berberine has the effect of lipid-lowering, but weaker than the effect of simvastatin, while berberine is characterized by lowering glucose with lipid-lowering.