Expression Of P16 And Kiss-1 In Colorectal Carcinoma And Its Significance

Objective:To explore the relationship between expression of tumor suppressor metastasis gene p16 and tumor suppressor gene Kiss-1 and their biological characteristics in colorectal cancer.Methods:The expression of p16 and Kiss-1 mRNA was determined by RT-PCR method in 50 cases of colorectal cancer and 10 adjacent normal mucosa.The expression of p16 and Kiss-1 proteins was determined by immunohistochemical Elivison methods.Results:1.RT-PCR results:The expression quantity of p16 and Kiss-1 mRNA was significantly lower in colorectal cancer than that in adjacent normal mucosa (P<0.05).The expression quantity of p16 and Kiss-1 mRNA were correlated with the Broders grading and Dukes staging of colorectal cancer, and it was significantly correlated with lymph node metastasis (P<0.05).There was positive correlation between p16 and Kiss-1 mRNA expression.2.Immunohistochemistry results:The expression rates of p16 protein were 48% and 100% in colorectal cancer and adjacent normal mucosa, and Kiss-1 protein was 56% and 100%, respectively.The expression rate of p16 and Kiss-1 protein was correlated with the grading and staging of colorectal cancer. The expression of p16 and Kiss-1 protein was significantly correlated with lymph node metastasis (P<0.05).3.The expression of p16 and Kiss-1 mRNA and protein was not correlated with age, gender and gross type.Conclusions:1.The p16 and Kiss-1 genes plays an important role in colorectal cancer genesis.2. The lower expression of p16 and Kiss-1 mRNA protein was correlated with infiltration and metastasis in colorectal cancer.3. Combination determine of p16 and Kiss-1 genes might be useful in indicating the prognosist in patients with colorectal cancer.