Relationship Between The Expression Of RASSF1A And CyclinD1, Apoptosis In Colorectal Cancer

Objective:Through detecting the expression rate of RASSF1A,cyclin D1 protein and apoptosis in colorectal cancer,we can investigate the relationship between RASSF1A and cyclin D1 protein expression, and the relationship between RASSF1A protein expression and apoptosis in colorectal cancer,Then approach the etiological factor and pathogenesis of colorectal cancer.Method:Expression of RASSF1A and cyclin D1 protein in colorectal cancer was examined by immunohistochemistry(IHC).Apoptosis in colorectal cancer was exmamined by TUNEL technique.The result of the examinamtion was statistically analyzed in combination with clinical data and pathological parameters.Result:1. The RASSF1A protein expression rate:The expression rate of RASS1A protein is gradually decreasing from the normal colorectal mucosa to colorectal cancer tissues,and the expression rate of colorectal cancer tissues is significantly lower than that in colorectal adenoma and normal colorectal mucosal tissues, the difference was statistically significant(P<0.05).2. The RASSF1A protein expression rate of colorectal cancer is related to its Duke's staging and lymph nodes metastasis. The RASSF1A protein expression rate of Duke's staging C and D colorectal cancer tissue is lower than that in Duke's staging A and B cancer tissue (the difference has statistical significance,P<0.05); The RASSF1A protein expression rate of lymph nodes metastasis group is lower than that in no lymph nodes metastasis group (the difference has statistical significance,P<0.05); and is not related to patients age, gender, tumor size, locus, tissue differentiation and tissue typing.3. The expression rate of cyclin D1 in normal mucous membrane of colorectum, colorectal adenoma, colorectal cancer:The expression rate is increasing progressively in the three tissues,and the difference between colorectal cancer and paracancer normal tissue has statistical significance (P＜0.05).4 the expression rate of cyclin D1 protein in colorectal cancer is related to its tissue differentiation, Duke's staging and lymph nodes metastasis, the cyclin D1 protein expression of well differentiated adenocarcinoma is lower than that of moderately differentiated adenocarcinoma and poorly differentiated adenocarcinoma,and the difference between well and poorly differentiated adenocarcinoma has statistical significance(P<0.05); the cyclin D1 protein expression of Duke's staging A and B colorectal carcinoma is lower than that of Duke's staging C and D(the difference has statistical significance,P<0.05); the cyclinDl protein expression rate of lymph nodes metastasis group is higher than no lymph nodes metastasis group (the difference has statistical significance, P<0.05); and is not related to patients age, gender, tumor size, locus, and tissue typing.5.The relationship between RASSF1A and cyclin D1 protein expression is a negative correlation in colorectal cancer(r=-0.313, P＜0.05).6.The AI(apoptosis index) in colorectal cancer:The AI of the RASSF1A protein positive expression group in colorectal cancer is significantly higher than that of the negative group(P<0.01).Conclusion:Colorectal cancer and adenomas exist RASSFIA protein expression loss and increased cyclin D1 protein expression, and the expression pattern is association with Duke's staging and lymph node metastasis, furthermore, the cyclin D1 protein expression increased with the degree of tumor differentiation, suggesting that RASSFIA and cyclin Dl protein closely related to the colorectal tumorigenesis. RASSFIA could inhibit the accumulation of cyclin D1 and promote cell apoptosis. Combine detection of RASSFIA and cyclin D1 maybe useful in diagnosis, prognosis and treatment to colorectal cancer.