Clinical Study On The Expression Of KAI-1and RASSF1A In Human Gastric Cancer

Objective: Gastric carcinoma as the most common cancer ingastrointestinal tumors,there are934thousand cases new cases each yearworldwide,42%(nearly40million) the number of new cases is in China, thesituation has become very serious.But now the exact pathogenesis of gastriccarcinoma is unclear,with the widely application of molecular biology,molecular genetics of cancer have been gradually understood。At present, hasconfirmed that the occurrence of gastric carcinoma is more than a gene factorand multi-step complicated process.In this experiment,to investigate the expression of KAI-1/CD82andRASSF1A in gastric cancer and metastatic lymphnodes, discuss thesignificance of KAI-1and RASSF1A play in the progression and metastasisof gastric cancer. To bring up a new targeted for the diagnosis and thetreament of gastric cancer, also help to judge the prognosis.Methods: Immunohistochemical(streptavidin-peroxidase,SP) techniquewas applied to detected the expression of KAI-1/CD82and RASSF1A in50cases of gastric carcinoma,50cases of the proximal adjacent tissues and20cases of normal gastric mucosa,and the comprehensive analysis of combinedwith the patient's age, gender, infiltration degree, lymph node metastasisclinicopathological factors and so on.Using the statistical software SPSS13.0statistical data processing, countwith χ2test, P <0.05is statistically significant. It analysed the relationshipbetween the expression of both in gastric carcinoma by using Spearman rankcorrdlationg which is one of non-parameter statistics.Results: The results of Immunohistochemistry showed that1①In50cases of gastric cancer, KAI-1/CD82showed that6ones werepositive,44ones were negative. In the adjacent tissue,the positive were35 15ones were negative. In20cases of normal gastric tissues,19ones werepositive,1ones were negative. There was very significant difference of thepositive rate of the expression between the cancer and normal tissues (P<0.05).②T he frequency of KAI-1/CD82was significantly lower in gastric tumorswith lymphatic metastasis than that without lymphatic metastasis (P<0.05),andin advanced stage (Ⅲ-Ⅳ)than in earlier stage (Ⅰ-Ⅱ)of the gaetric cancer(P<0.05).However,there was no significant difference of the frequency ofkai-1/CD82when stratefied by age,sex,histological type as well asdifferentiated degree of patients of GC (P＞0.05).2①In50cases of gastric cancer, RASSF1A showed that8ones were positiveand42ones were negative. In the normal gastric tissues,18ones werepositive and2ones were negative. There was very significant difference ofthe positive rate of the expression between the cancer and normal tissues(P<0.05).②The frequency of RASSF1A was significantly lower in gastrictumors with lymphatic metastasis than that without lymphatic metastasis(P<0.05),and in advanced stage (Ⅲ-Ⅳ)than in earlier stage (Ⅰ-Ⅱ)of thegaetric cancer (P<0.05).However,there was no significant difference of thefrequency of RASSF1A when stratefied by age,sex,histological type as wellas differentiated degree of patients of GC (P＞0.05).3The relationship between the expression of KAI-1and RASSF1A in gastriccarcinoma Using Spearman rank correlation analysis indicated that the KAI-1and RASSF1A expression in gastric carcinoma was positively correlated (rs=0.544, P <0.05).Conclusion: The function status of KAI-1and RASSF1A may be closelyassociated with the invasion and metastasis of gastric cancer. It may be anew significant biological markers that can reflect invasion, metastasis andprognosis of gastric cancer, which also may buring up a significant clinicalmeaning for us to early detecting and prevent happening. meanwhile,KAI-1and RASSF1A shows a new mechanism of metastasis of gastric cancer,that provides a new way for confronting metastasis of gastric cancer.