| With the increasing of the elderly population in the world, there are different degree aging problems, which gradually become an obvious attention in many countries and society. In aging process, heart is earlier than other organs, which appears degenerative changes in the structure and function, seriously affecting the health of the elderly, while the dynamic balance of production and scavenging of free radicals to break, is considered one of the important reasons of myocardial aging. There are many interventions delaying myocardial aging, and sport, diet is best. In this study, rats were injected D-galactose to copy subacute aging model, imposing weight training and soybean peptides supplement in 6 weeks modeling process. We observed changes of serum and myocardial indicators in aging rats and analyze the effects of myocardium antioxidant capacity, in order to accumulate a certain theoretical basis and experimental reference for the future crowd experiments, but also promote effective sports pattern, rational nutritional supplement and the new method of combining sport with nutrition to provide theoretical basis and practical guidance in the elderly population.In this study, 56 three-month-old SD male healthy rats were randomly divided into 7 groups: control group (C), model group (M), small load exercise group (S), big load exercise group (B), peptide group (P), small load exercise and peptide group (SP), big load exercise and peptide group (BP). Except group C, the other groups were interfered respectively treadmill weight training and the soybean peptides in 6 weeks modeling process. After 6 weeks, all groups were killed to compare the serum and myocardial biochemical markers.Results1. After 6 weeks, compared with group C, the serum MDA level was higher significantly (P<0.05), and SOD vitality was very lower significantly in group M (P<0.01).2. After 6 weeks, compared with group C, myocardium MDA and lipofuscin level were very lower significantly in group M (P<0.01, P<0.01). Weight training made myocardium MDA and lipofuscin level of aging rat decrease significantly (P<0.05, P<0.05); Soybean peptides supplement made myocardium MDA and lipofuscin level of aging rat have declining trend (P>0.05, P>0.05), and they had significant or very significant interaction (P<0.05, P<0.01).3. After 6 weeks, compared with group C, myocardium SOD, CAT, GSH-PX vitality were very lower significantly in group M (P<0.01, P<0.01, P<0.01). Weight training or soybean peptides supplement made myocardium SOD, GSH-PX vitality of aging rat decrease significantly (P<0.05, P<0.05), and they had significant interaction (P<0.05, P<0.05). Weight training or soybean peptides supplement made myocardium CAT vitality of aging rat have increased trend (P>0.05, P>0.05), but they had significant interaction (P<0.05).4. After 6 weeks, compared with group C, myocardium NO level was very lower significantly in group M (P<0.01). Weight training or soybean peptides supplement made myocardium NO level of aging rat have increased trend (P>0.05), but they had significant interaction (P<0.05).5. After 6 weeks, compared with group C, myocardium Na+-K+-ATPase and Ca2+-Mg2+-ATPase vitality were very lower significantly in group M (P<0.01, P<0.01). Weight training made myocardium Na+-K+-ATPase vitality of aging rat increase significantly (P<0.05), and soybean peptides supplement made myocardium Na+-K+-ATPase vitality of aging rat have increased trend (P>0.05), but they had significant interaction (P<0.05). Weight training or soybean peptides supplement made myocardium Ca2+-Mg2+-ATPase vitality of aging rat very increase significantly or increase significantly (P<0.01, P<0.05), and they had significant interaction (P<0.05)Conclusions1. Through the 6 weeks D-galactose subcutaneous injection, the serum MDA level increased and SOD vitality decreased , and myocardium lipofuscin level increased, as well as the appearance of aging symptoms and behavioral changes in group M. These indicated the rat aging model can be copied successfully.2. 6 weeks weight training or soybean peptides could enhance the different levels of myocardial antioxidant enzymes in aging rat, and reduce the generation of lipid peroxidation. Combination weight training with soybean peptides had notable significant interaction to enhance myocardial antioxidant capacity of aging rat, in which small load exercise and peptide group was best. And antioxidant appear comprehensive performance: myocardium SOD, CAT, GSH-PX vitality and NO level increased , and MDA and lipofuscin level decreased.3. 6 weeks weight training or soybean peptide supplement could improve myocardium Na+-K+-ATPase and Ca2+-Mg2+-ATPase vitality of aging rat. Combination weight training with soybean peptides had notable significant interaction to enhance myocardial Na+-K+-ATPase and Ca2+-Mg2+-ATPase vitality, reduced free radical damage to cell membranes, maintain cell membrane stability, enhanced ion transport,regulated energy metabolism and delayed myocardium aging. |
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