Compatible Regulation And Mechanism Of Resisting Chronic Renal Failure Of Qize Decoction

Objective:To investigate the compatible regulation of Qize decoction by observing the effects of Qize decoction and its disassembled compositions on resisting chronic renal failure.To discover the effective combination from the Qize decoction and its disassembled compositions. To explore the mechanism of resisting chronic renal failure of the effective combination and provide the experimental reference for the clinical.Methods:Choosing the digital form L8 (26) of orthogonal design which ignoring the interaction of the different herbs, the Qize decoction was disassembled into 8 groups: the whole prescription, the Huangqi-Gegen-Zelan group, the Dahuang-Zelan group, the Huangqi-Dahuang-Yimucao group, the Huangqi-Niubangzi group, the Yimucao-Gegen group, the Dahuang-Niubangzi-Gegen group, the Niubangzi-Yimucao-Zelan group.90 male Sprague Dawley(SD)rats were randomly divided into eleven groups:the blank control group(8 rats),the model group(10 rats),the Niaoduqing group(8 rats) and the eight disassembled groups(8 rats every group). Every rat was treated with 2% adenine in the morning and stomached its group correspondence decoction in the afternoon for 28 days except the blank control group. Common physical change of the 11 groups rats were observed. Blood urea nitrogen (BUN) and serum creatinine (Scr) of those were detected. Pathological change of the experimental rats'renal tissue were observed by Hematoxylin and eosin staining (HE) method. The protein expression of transforming growth factor-β1 (TGF-β1) and Thrombospondin-1(TSP-1) in the experimental rats'renal tissue were examined by immunohistochemistry.Results:①The change of weight in every group:The rats'weight in the model group, the Dahuang-Zelan group,the Dahuang-Niubangzi-Gegen group and the Niubangzi-Yimucao-Zelan group increased with the time in the first three weeks, and began to decline in the fourth week, especially the model group. On the other side, the rats'weight in the rest groups increased with the time during the four weeks.②The change of kidney function:Compared with the model group, the level of BUN, Scr in the Niaoduqing group, the whole prescription group, the Huangqi-Gegen-Zelan group, the Huangqi-Dahuang-Yimucao group and the Yimucao-Zelan group were lower (P<0.05).③The renal histomorphological change in every group:Compared with the model group, the kidney weight, renal index in the rest groups decreased significantly (P<0.05) except the Huangqi-Niubangzi group. Compared with the model group, the pathological damage in the other groups were lighter (P<0.01), especially in the whole prescription group and the Niaoduqing group.④The protein expression of TGF-β1, TSP-1:There were little TSP-1 protein expression and low TGF-β1 protein expression in renal tissue in the blank control group, while high protein expression of TGF-β1 and TSP-1 in the model group. Compared with the model group, the protein expression of TGF-β1 and TSP-1 in renal tissue in the rest groups except the Dahuang-Niubangzi-Gegen group were lower (P<0.01), and the protein expression of those in the Niaoduqing group, the whole prescription group, the Huangqi-Gegen-Zelan group and the Huangqi-Dahuang-Yimucao group were lower significantly.Conclusion:The whole prescription group, the Huangqi-Gegen-Zelan group and the Huangqi-Dahuang-Yimucao group could protect the renal function of adenine-induced CRF rats, depress the pathological damage and delay the process of CRF. Combination of qi-tonifying herbs, damp-draining diuretic, blood-activating herbs might improve the CRF effectively. The Qize decoction could improve CRF by reducing the protein expression of TGF-β1 and TSP-1 in the renal tissue.