Dynamic Changes Of Lymphocyte Subsets In Children With Epstein-Barr Virus Infection

ObjectiveTo study the changes of lymphocyte subsets in children with Epstein-Barr virus (EBV) infection, and to explore whether there is any correlation between lymphocyte subsets and anti-EBV-VCA-IgM (EBV-viral capsid antigen-IgM) titer.Methods147 cases of EBV-infected children for observation group,48 cases of exclusion herpes virus such as EBV-infected children as control group subjects. According to different forms and different age, the 147 cases of EBV-VCA-IgM positive infections children were divided into two groups:only EBV infection, infectious mononucleosis (IM),<3 years old and≥3 years old. The antigen on the surface of peripheral blood mononuclear cells including CD3+, CD8+, CD4+, CD56+, CD19+ was analyzed by flow cytometry, compared with the control group. At the same time, EBV-VCA-IgM titer were detected and the correlation between the titer and cell subsets were calculated.Results1.In the group of EBV infection, CD4+, CD56+ decreased, CD19+ increased. Significant changes were found in IM group:CD8+ was higher than controls and the group of merely EBV infection, CD4+, CD56+ decreased obviously, which is lower than the other two groups, ratio of CD4+/CD8+ inverted, CD19+ decreased significantly.2.The group of<3-year-old, CD3+, CD4+ was less than the control group, CD19+ increased than the control group and≥3-year-old group, no statistical difference in CD8+, CD56+. Compared with the controlled group,≥3-year-old group had the obvious changes, CD3+, CD8+ was significantly higher than the controlled group and <3-year-old group, CD4+, CD56+, which were lower obviously than the other two groups, ratio of CD4+/CD8+ inverted. CD19+ only lower than the<3-year-old group. 3.CD8+ and EBV-VCA-IgM titers were positive correlated, CD4+, CD4+/CD8+, CD19+ showed significantly negative correlation, while the CD56+ and EBV-VCA-IgM titers had no correlation.4.After the clinical symptoms improved, two cases of IM group were reviewed for their lymphocyte subsets, which showed that CD3+, CD8+ decreased, CD4+, CD19+ increased.ConclusionsCompared with the younger, merely EBV infected children, the lymphocyte subsets change more obviously in the older age, IM children. There was significant correlation between lymphocyte subsets and EBV-VCA-IgM titer, which can predict the degree of cellular immune dysfunction...