| ObjectiveBy acute exacerbation of asthma and bronchiolitis in children with serum 25-hydroxy vitamin D3 in serum, serum 25-hydroxy vitamin D3 levels and acute attack of asthma and the relationship between bronchiolitis for children with breathing disorders treatment of new theoretical and clinical basis.Subjects and Methods1. the study(1) Asthmatic group:December 2009-March 2010 Shengjing Hospital of China Medical University Pediatric Respiratory Medicine Ward 18 cases of hospitalized children in line with the Chinese Academy of Pediatrics study group of children with bronchial asthma, respiratory control of conventional diagnostic criteria of asthma in children raised, aged 10 months to 5 years with an average 3.12 years, with 12 males and 6 females.(2) Bronchiolitis group:the same period in pediatric respiratory medicine in our hospital 24 patients hospitalized children, aged 2 months to 3 years with an average 9.98 months, consistent with the sixth edition of "Pediatrics" clinical diagnosis of bronchiolitis,17 cases were male,7 females, the mother no smoking, full-term children, except, except in respiratory tract, respiratory tract abnormalities and bronchial lymph node tuberculosis, caused by breathing, and except for a history of allergic diseases and related family history.(3) The control group:children from the hospital over the same period 20 cases of children hospitalized orthopedic surgery, who had no asthma, allergies and related family history, history, history of the past 4 weeks, no infections, no history of active rickets, aged 8.5 months to 5 years, mean 2.44 years, of which 10 males and 10 females.2. the main instruments and reagents(1) Instruments:centrifuges,-70℃refrigerator, automatic plate washer, automatic microplate reader, ELIS A Kit(2) Reagents:25-hydroxy vitamin D3 enzyme immunoassay kit (Takara Biotechnology Co. Ltd. Crystal)3. method(1) Acquisition history:the time of admission in children with allergic asked in detail about personal history, family history of allergies, past history of wheezing, wheezing with or without history of common cold.(2) Specimen collection:children admitted to hospital for treatment when the pre-fasting venous blood collected 1ml, centrifugal 1000r/min×3 minutes, collecting serum rear a 70℃refrigerator cold, and healthy control group with the same specimens.(3) Detection①Remove the ELISA plate, in accordance with the order corresponding to the standard were added to 1 OOul in the blank in Porous Media②sample numbers were marked by adding 100ul of the sample in the micro blank.③In the standard bore holes and sample by adding 50ul of enzyme marker solution④36±2℃incubated for 60 minutes reaction⑤plate washer washing five times, each time put it aside for 10-20 seconds⑥by adding at the end of each hole objects A, B solution of 50ul⑦36±2℃under dark incubation reaction for 15 minutes⑧for each hole by adding 50ul stop solution to terminate reaction.(4) The results to determine:①Instrument value:a microplate reader at wavelength of 450nm to read the value of the Conde OD②B= standard OD values, BO= standard 0:00 of the OD values of③B/BO% value of the vertical axis, to standard concentration of abscissa in the logarithmic coordinate paper, the standard curve drawn④in the standard curve to find the corresponding concentration range.(5) statistical analysis Using SPSS software for statistical analysis, the results indicated that using mean±standard deviation, the application t test, P<0.05 statistically significant.Results1. acute asthma serum 25-hydroxy vitamin D3 levels compared with the control groupAcute attack of asthma in children with serum 25-hydroxy vitamin D3 levels were significantly lower than the control group, in comparison with a significant difference, t value of 2.924, P＜0.006.2. children with bronchiolitis of serum 25-hydroxy vitamin D3 levels compared with the control groupBronchiolitis serum 25-hydroxy vitamin D3 levels were significantly lower than the control group, in comparison with a significant difference, t value of 2.694, P <0.013. asthma and bronchiolitis serum 25-hydroxy vitamin D3 deficiency rate25-hydroxy vitamin D3 serum of normal for the 11ng/ml-60ng/ml. Asthma group,18 patients,25-hydroxy vitamin D3 decreased in 6 cases, accounting for 33.34%; 24 cases of bronchiolitis,25-hydroxy vitamin D3 decreased in 8 cases, accounting for 33.34%.4. acute attack of asthma in children with bronchiolitis and serum 25-hydroxy vitamin D3 levels betweenAcute attack of asthma in children with bronchiolitis and serum 25-hydroxy vitamin D3 was no difference compared to the level, t value of-0.297, P> 0.5, comparison between the two there is no significant difference. Conclusion1. asthma in children with acute exacerbation of serum 25-OH-D3 were significantly lower than control group children.2. children with bronchiolitis serum 25-OH-D3 were significantly lower than control group children.3.33.34% of the asthmatic children in acute attack of serum 25-OH-D3 levels decrease,33.34% of children with bronchiolitis also found in serum 25-OH-D3 levels decreased.4. acute exacerbation in asthmatic children and children with bronchiolitis levels of serum 25-OH-D3 levels were not significantly different. |
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