Background:The prevalence rate of Diabetes mellitus (DM) is rising steadily in the world, and DM has become one of the three diseases threatening people's health and life. However, the mechanism of DM, especially T2DM is not clear. There is an inflammation hypothesis, insisting that T2DM is a chronic and low level systemic disease, in which many cytokines play important roles. More and more attention has been given to the relationship of cytokines, T2DM and its complication. PANDER (PANcreatic DERived factor, FAM3B) is a noval cytokine, highly expressing in isletβcells. High dose of PANDER inducesβcells apoptosis, inhibits insulin secreting and aggravates insulin insistance, indicating that PANDER may be reletated to T2DM.Objective:To determinate the level of serum PANDER in newly diagnosed type 2 diabetic patients and to investigate the relationship between isletβcell function, insulin resistance and serum PANDER.Method:We studied 36 patients with newly diagnosed type 2 diabetes in the department of endocrinology of Shandong Provincial Hospital (T2DM group).18 males,18 females, aged beween 20 years old and 70 years old. The patients were diagnosed according to the diagnostic criteria for diabetes developed by WHO in 1999. There were some exclusion criterions:history of taking hypoglycemic drugs and injecting insulin, with complicating disease of DM, hypertension, dyslipidemia, coronary heart disease, hepatic and renal diseases, autoimmune diseases, tumor, disease of intestine/testis/prostate, disease of neurons of central nervous system.23 normal participants as controls (NC group), male 10, female 13, aged beween 27 years old and 61 years old. There were some exclusion criterions:DM, hypertension, dyslipidemia, coronary heart disease, hepatic and renal diseases, autoimmune diseases, tumor, disease of intestine/testis/prostate, disease of neurons of central nervous system, long drug history. Oral glucose tolerance and insulin releasing test (OGIRT) were performed in the two groups. General information, physical examination and biochemical data were collected. Serum PANDER was measured by ELISA kit, blood glucose and blood lipids by automatic biochemical analyzer in all subjects, blood insulin by Chemiluminescence. Isletβcell function was evaluated by HOMA-βindex, MBCI; insulin resistance was evaluated HOMA-IR index, IAI, Matsuda-ISI (WBISI). All datas were processed by statistic package deal SPSS 16.0. Variables of normal distribution were shown asχ±s. t test was used to compare the two groups. Linear correlation analysis was used. A P value less than 0.05 was considered indicative of statistical significance.Result:1. The serum PANDER level, fasting plasma glucose (FPG), increment area under the glucose curve (AUCG), fasting plasma insulin (FPI), increment area under the insulin curve (AUCI), HOMA-IR of T2DM group were significantly higher than those of the NC group (P< 0.05); HOMA-βindex, IAI and WBISI were significantly lower than those of the NC group (P< 0.01); MBCI of two groups was not different significantly (P>0.05).2. Serum PANDER was independently positively correlated with isletβcell function (P< 0.05), not correlated with insulin resistance (P>0.05) in T2DM group. Conclusion:1. PANDER may be a novel serum inflammatory marker.2. There is a close relationship between PANDER and isletβcell function; PANDER may take part in the self-injury of isletβcell and the pathogenesis of T2DM. |