The Correlated Research About Il-1β,pge2 With Insulin Section And Function In Type 2 Diabetes Mellitus

Objective:To investigate the concentration of plasma interleukin-1β(IL-1β) and prostaglandinE2(PGE2) levels in the population with different glucose tolerance,and then analyze the relationship and possible pathogenesy between plasma IL-1β,PGE2 with insulin resistance (IR) and pancreatic isletβcell destruction.Methods:(1)We selected 200 cases from the first affiliated hospital of medical school Shihezi University between December 2008 and August 2009 on basis of the diagnostic criteria and typing of diabetes mellitus in 1999.100 cases were type 2 diabetes mellitus (T2DM),100 cases were impair glucose tolerance (IGT), 60 cases healthy people were selected as normal controls (NC).And then all cases were separated non-obese subgroup (BMI<25Kg/m2) and obese subgroup (BMI>25Kg/m2) T2DM cases were separated course greater than 5 years subgroup and course less than 5 years subgroup.(2)plasma PGE2,IL-1βwere measured by radiate immune approach.(3)Calculated insulin resistance index(homeostasis model assessment HOMA-IR) and pancreatic isletβcell function index(homeostasis model assessment HOMA-β).(4)Continuous variables were presented as mean±sd and difference between two groups were tested using t-test and analysis of variance(ANOVN),to assess the dependability by coefficient of relate/ partial correlation and multiple regression analysis.Results:Fasting blood plasma PGE2:T2DM group (63.99±17.48pg/ml),IGT group(55.69±15.00pg/ml) were higher than NC group(49.41±15.90pg/ml), T2DM group was higher than IGT group too, there were significantly difference (p<0.05), course greater than 5 years subgroup (69.54±15.10pg/ml) was higher than course less than 5 years subgroup (58.45±18.08pg/ml), significantly difference (p<0.05). It is not significantly difference (p>0.05) in obese subgroup (51.48±16.25pg/ml) and non-obese subgroup (47.19±15.48pg/ml) of NC. obese subgroup of IGT(64.70±17.77pg/ml) was higher than non-obese subgroup (45.53±10.04pg/ml), significantly difference (p<0.05).obese subgroup of T2DM (72.83±14.77pg/ml) was higher than non-obese subgroup (53.63±14.56pg/ml), significantly difference (p<0.05).Fasting blood plasma IL-1β:T2DM group (0.41±0.17ng/ml) was higher than IGT group (0.36±0.16ng/ml) and NC group (0.32±0.10ng/ml), and significantly difference (p<0.05), IGT group was higher than NC group, but no significantly difference (p>0.05);course greater than 5 years subgroup (0.44±0.17ng/ml)was higher than course less than 5 years subgroup (0.39±0.16ng/ml), it is significantly difference (p<0.05). It is not significantly difference (p>0.05) in obese subgroup (0.34±0.08ng/ml) and non-obese subgroup (0.29±0.11ng/ml)of NC. obese subgroup(0.46±0.10ng/m) of IGT was higher than non-obese subgroup (0.24±0.12ng/ml)significantly difference (p<0.05). obese subgroup (0.50±0.10ng/ml) of T2DM was higher than non-obese subgroup (0.31±0.17ng/ml) significantly difference (p<0.05).The result of correlation analysis:HOMA-IR index was positively correlated with PGE2, IL-1β(p<0.05), HOMA-βindex was negatively correlated with PGE2, IL-1β(p<0.05).The result of multiple regression analysis show that PGE2, IL-1βwere risk factors of IR and pancreatic isletβcell function destruction. Conclusion:(1) The concentration of plasma PGE2, IL-1βwas higher in IGT, T2DM patients. Chronic inflammatory reaction may play an important role in the process of T2DM. (2) PGE2, IL-1βare risk factors of IR and pancreatic isletβcell function. The level of them could reflect the degree of IR and pancreatic isletβcell function destruction indirectly. (3) PGE2, IL-1βtook part in IR and pancreatic islet p cell function destruction, the pathogenesy which was unknown need to search.