| Autogenous tooth transplantation, or autotransplantation,is to extract the integrity of teeth by surgical methods, and transplant them to other teeth-missing parts of its own, and exercise functions.Although many literature reported that the survival rate of transplanted teeth could be as high as 90%, but there were still many cases showing bone adhesion of the periodontal ligament healing methods.Recent studies have shown that stimulation of early chewing will reduce the incidence of bone adhesion, and early loading of suitable force controlled by orthodontic devices will enhance the success rate of transplanted teeth.This study selected alkaline phosphatase (ALP) and basic fibroblast growth factor (bFGF) as indicators of periodontal tissue alterations. Real Time PCR method was adopted.The fluorescence of PCR product was used to quantitate the mRNA expression of ALP and bFGF,so that to evaluate the proliferation and differentiation capabilities of fibroblasts and osteoblasts in periodontal tissues,to study the effect on autotransplanted teeth periodontal tissues prognosis with orthodontic loading.ObjectiveThis study aimed to establish animal models of autotransplanted teeth with orthodontic loading of different start time/different time course, and evaluate the effect on autotransplanted teeth periodontal tissues prognosis with orthodontic loading systematically through detecting the expression variability of ALP and bFGF in periodontal tissues by real-time quantitative PCR.We hope to explain the beneficial and adverse effects of orthodontic treatment on the healing process of autotransplanted teeth, and provide references for grasping the start time,the force value,the displacement distance, the duration of loading in clinical practice.Methods12 healthy adult male Beagle dog were selected as the object of study. Autogenous teeth transplantation was done after anesthesia by using 2% ketamine to induce, and 3% pentobarbital sodium to do intravenous injection.Both sides of the maxillary first premolars were chosen as the donor teeth.Teeth were extracted carefully,then exchanged and transplanted the tooth of one side to the contralateral tooth socket which had been prepared to fit the donor teeth by planting machine. Ligated transplanted teeth with the adjacent teeth.Dismantled the fixed equipments and undertook conventional root canal therapy after one week.Loaded (3oz) the left maxillary transplanted teeth respectively at the first,fourth,eighth and twelfth week.Made the unloaded right maxillary transplanted teeth and the loaded left mandibular first premolars as experimental control groups,while the right mandibular first premolars as blank control groups.After loading for 1,2,4 weeks,animals were killed respectively to obtain the necessary teeth and surrounding periodontal tissues.Selected alkaline phosphatase (ALP) and basic fibroblast growth factor (bFGF) as indicators of periodontal tissue remodeling.In this study, Real Time PCR method was adopted. The fluorescence of PCR product was used to quantitate the mRNA expression of ALP and bFGF, accordingly differences of prognosis of autotransplanted teeth with orthodontic loading of different start time/different time course were compared.ResultsModels of autotransplanted teeth with orthodontic loading on Beagle dogs were established successfully. No matter with or without orthodontic loading, there were no abnormal loosening and dislocation on autotransplanted teeth.However, according to pre-and postoperative periodontal examination by probing,we found that the periodontal pocket of autotransplanted teeth, whether loaded or not,were all deeper than pre-operation. Through quantitative analysis of the mRNA expression of ALP and bFGF in periodontal tissues of autotransplanted teeth by Real Time PCR,we found that the mRNA expression levels of ALP and bFGF of autotransplanted teeth with orthodontic loading were higher than the unloaded teeth(P<0.05).The mRNA expression differences of ALP and bFGF in periodontal tissues of autotransplanted teeth with orthodontic loading of same start time/different time course were statistically significant (P<0.05).The mRNA expression differences of ALP and bFGF in periodontal tissues of autotransplanted teeth with orthodontic loading of different start time/same time course were statistically significant (P<0.05).Conclusions1. Choosing the first premolar teeth of Beagle dogs as donor teeth of autotransplantation, we can establish a simple and reliable animal model of autotransplanted teeth. The animal model can provide a good clinical reference value.2. The orthodontic loading after autologous teeth transplantation contributes to the rebuilding of alveolar bone and can prevent ankylosis.3. The rebuilding of alveolar bone of autotransplanted teeth with orthodontic loading is the most active when loading continues for 2 weeks.4. The orthodontic loading after autologous teeth transplantation fourth to eighth week can improve the prognosis of transplanted teeth. |
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