Effects Of Sanpi Decotion On Experimental Mice With Ulcerative Colitis And The Expression Of IL-2 And IL-10

Objective: To investigate the therapeutic effect of sanpi decotion in ulcerative colitis(UC) mice and discuss its therapeutic mechanism.Methods: Forty BALB/C mice were divided randomly and equally into normal group, model group, sulfasalazine (SASP) group, and sanpi decotion group, ten mice in each group. After set up the oxazolone colitis, SASP group and sanpi decotion group were treated with sulfasalazine and sanpi decotion respectively, to observe the weight,stool,food and drink situation of the mice every day. Killed all the mice on the tenth day, separated the spleen and weight it, got the colon and evaluated the histological changes of the damaged colonic tissues, and measured the interleukin(IL)-2 and IL-10 concentrations in serum by using the enzyme-linked immunosorbent assay(ELISA).Result: 1. The symptom of sanpi decotion group and SASP group UC mice had great amelioration compared to model group mice2.The immune organ spleen of the model group mice was conspicuous shrunk, and compared to model group, the weight of spleen in sanpi decotion group and SASP group was increased.3. The concentration of IL-10 in the serum was markedly increased in model group, and the expression of IL-2 was decreased, oral administration of sanpi decotion significantly increased the expression of IL-2, and down regulation the expression of IL-10 in the serum compared to the model group.Conclusion: 1. Sanpi decotion can certainly treat the UC model mice.2. Sanpi decotion can ameliorate the ulcerative colitis by regulate the balance of the cytokines, improve the function of immunity, and control the inflammation in the colon.