Prepare Ultra-flexible Nanoliposomes Of Indomethacine And Study The Effect Of Ultra-flexible Nanoliposomes On Diffusion Rate Of Indomethacine Through The Skin Of Rats

OBJECTIVE To prepare ultra-flexible nanoliposomes of indomethacine and study the effect of ultra-flexible nanoliposomes on diffusion rate of Indomethacine through the skin of ratsMETHODS Membrane-sonication method was used to prepare ultra-flexible nanoliposomes.The use of high-performance liquid chromatography(HPLC) determination of indomethacin encapsulation efficiency(EE%) and drug loading(DL%).Indomethacine permeation tests in vitro through the skin of rats were performed in Franz diffusion cells.RESULTS Indomethacin ultra-flexible nanoliposomes particle size distributed uniformly.The concentration of indomethacine from linear range of detection by HPLC method was 1-50μg / ml.Preparation of flexible nano-liposomes encapsulation efficiency was(81.07±0.34)%, drug loading was(5.78±0.32)%,average particle size was(64.54±3.12) nm.Nano-liposomes as a carder could increase the indomethacin penetration rate;Compared with ordinary nanoliposomes,ultra-flexible nanoliposomes could increase diffusion rate of Indomethacine through the skin of mice.CONCLUSIONS The Ultra-flexible nano-liposomes preparation method is feasible,the analysis system in vitro is stable.Received indomethacine Ultra-flexible nanoliposomes are reasonable,high encapsulation efficiency,and reliable performance.Ultra-flexible nanoliposomes can significantly enhance the diffusion rate of Indomethacine through the skin of rats.