Effect Of Exogenous L-carnitine On Activities Of Respiratory Chain Complexes In Mitochondria From Mice's Liver And Free Radical Metabolism

The human body depends mainly on fatty acid oxidation for energy under the condition of Long time and low-intensity exercise,The Carnitine as a carrier sends long chain fatty acid from the outer membrane of mitochondrion into the inner in the form of acyl-carnitine to make fatty acidβ- oxidized.Mitochondria is the place where the ATP generated,ATP is the direct source of the organic energy.The research aims to mainly discuss whether the added carnitine has positive influence on liver metabolism of the big rat,which is experimented by means of adding L-carnitine to the exercising rat and observe its effect on the enzyme activity of the rat's liver mitoch-ondrial respiratory chain and free radical metabolism,so as to provide the basis for this statement and the basic information for scientific research further.Experiment methodsChoose 50 male healthy Whister mice of two-month old,Body weight about 120±10g.After one week,arbitrarily divide them into following groups:Control Group(C,n=10),carnitine complement(L,n=10),training(T,n=10),training +carnitine complement(TL,n=10).The mice of the first two groups don't any exercise,while the rest two do running with progressive load for six weeks.The groups of L and TL are food with carnitine(300mg/kg·bw),between eight and nine o'clock every morning while the groups of C and T are food with distilled water of the same amount.After six weeks training kill the mice of C,L,T,and TL when exhause,then measure the weight,time of exhause,activity of mitochondrial respiratory chain compoundⅠ,Ⅱ,Ⅲ,Ⅳ,SOD,MDA,GSH-PX and the change of each index.Experiment conclusions1 Supplementation L-carnitine combined with exercise training can significantly reduce the growth of body weight in rats.2 Supplementation L-carnitine combined with exercise training in rats can be extended to exhaustive treadmill exercise time.3 Supplementation L-carnitine in rats after exhaustive treadmill immediately to the liver mitochondrial respiratory chain activity had no effect on CⅠ;exercise training can make the CⅠhave significantly increased activity,exercise training and supplementation carnitine in rats can run to Units immediately after exhaustive liver mitochondrial respiratory chain activity of the CⅠhas obvious synergies.4 Simple and pure L-carnitine on exercise training in rats after exhaustive treadmill immediately to the liver mitochondrial respiratory chain had no effect on CⅡactivity, exercise training and L-carnitine can be added so that rats ran to exhaustion immediately after Units liver mitochondria CⅡrespiratory chain of no obvious synergies.5 Supplementation L-carnitine in rats after exhaustive treadmill immediately to the liver mitochondrial respiratory chain activity had no effect on CⅢ;exercise training can make CⅢactivity has significantly increased,exercise training and supplementation carnitine in rats can run to Units immediately after exhaustive liver mitocho n 1 dria 1 respiratory chain activity of CⅢno obvious synergies.6 Supplementation L-carnitine in rats after exhaustive treadmill immediately to the liver CⅣmitochond rial respiratory chain activity has significantly increased;exercise training can make the activity of CⅣhas significantly improved with the supplementation exercise training can make rats run carnitine Units Exhaustive immediately to the liver mitochond rial respiratory chain activity of CⅣhas obvious synergies.7 Supplementation L-carnitine can be added simply to make rats after exhaustive treadmill to instantly reduce the liver MDA content,but not significantly;SOD activity increased slightly but not significantly;GSH-Px activity has markedly improved.Exercise training can make rats exhaustive exercise to reduce the MDA content,SOD activity increased slightly but not significantly;GSH-Px activity has very markedly improved.Combination of exercise training can supplement carnitine to exhaustive exercise in rats the content of MDA significantly decreased,SOD and GSH-Px activity was significantly increased.Showed that endurance training and supplementation carnitine has antioxidant synergy.