Effects Of Captopril,Xin Fu Kang Oral Liquid On The Activities Of Complexes â… -â…£ In The Respiratory Chain Of Myocardium Mitochondria In Chronic Heart Failure Rats

Objective: The paper focuses on the effect of traditional Chinese medicine Xin Fu Kang Oral Liquid on cardiac function and the activities of complexesⅠ-Ⅳin the respiratory chain of myocardium mitochondria in pressure overload-induced left ventricular hypertrophy induced by abdominal aortic constriction in rats.Also it discusses the mechanism of the energy metabolism in CHF as well as the treatment of CHF.Methods: random allocation was taken to averagely divide 100 SD rats into four groups:Sham operation(SH),Coarctation of abdominal aorta model group (CAA),Captopril Tablets cure group(CAP) and Xin Fu Kang Oral Liquid group(XFK). Pressure overload-induced left ventricular hypertrophy induced by abdominal aortic constriction was used to prepare rat models with congestive heart failure. And then measure the parameters of cardiac function. The activities of complexes Ⅰ-Ⅳin the respiratory chain of myocardium mitochondria were measured by Shanghai GENMED CO,LTD.-supplied assay kit for the purpose of observing the index of the parameters of cardiac function (HR, SAP, DAP, LVMI, LVEDP,±dp/dtmax etc.)and the change of activities of complexesⅠ-Ⅳin the respiratory chain (NADH reducase,SDH, Co.Q Hz2Cyt.C reducase, CCO) after six and eight weeks respectively.Result: (1) The study shows that CAA group cardiac function decreased, LVMI,SAP,DAP,LVEDP increased, +dp/dtmax, -dp/dtmax decreased, and the activities of complexesⅠ-Ⅳdecreased,among which the decrease of SDH and CCO has significant meaning(P<0.01)。(2) In the XFK group and in CAP group all kinds of cardiac function parameters and the activities of complexesⅠ-Ⅳincreased compared with CAA group by the 10th and 12th week (P<0.01),and the increase of the SDH and CCO is more obvious (P<0.01).It is more obvious by the time of the 12th week, the increase of the SDH and CCO is obvious (P<0.01). (3) All the cardiac function parameters and the activities of complexesⅠ-Ⅳwere better at 12th week than at 10th week,and the increase of the SDH and CCO is more obvious (P<0.05). (4)In the XFK group,the increase of all kinds of cardiac function parameters and the activities of complexesⅠ-Ⅳis more obvious compared with CAP group by the 10th and 12th week, the increase of the SDH and CCO is more obvious(P<0.05,P<0.01).Conclusion:(1) No doubt, coarctation of abdominal aorta in rats causes pressure overload-induced left ventricular hypertrophy by which the model of Congestive Heart Failure can be prepared, as time goes by, heart failure is more serious for rats,cardiac function and the tested activities of complexesⅠ-Ⅳin the respiratory chain of myocardium mitochondria which can demonstrate the condition of myocardial energy metabolism obviously decrease. Captopril and traditional Chinese medicine Xin Fu Kang Oral Liquid on function of invigoration QI warming YANG and promoting blood circulation to removing blood stasis can obviously enhance and improve cardiac function of CHF rats and the activities of complexesⅠ-Ⅳ. Thus, the disfunction of Myocardial Energy Metabolism plays an important role in the pathogenesis and progress of CHF. Captopril and Xin Fu Kang Oral Liquid can obviously enhance the Myocardial Energy Metabolism of rats by improving the activities of complexesⅠ-Ⅳin the respiratory chain of myocardium mitochondria and this may be one of mechanisms for the treatment of CHF.So, to improve the metabolism in the myocardium energy metabolism for the treatment of CHF is one of the subjects for our future research and maybe the mechanism of treatment of CHF by traditional Chinese medicine is closely related to improvement of Myocardial Energy Metabolism.