Study On Quality Standard Of Semen Strychni Pulveratum And Fingerprint Of Semen Strychni

Semen strychni which posses the action of relieving swelling and stoping pain is a kind of drying mature seed of Loganiaceae strychnos sp. It has important value in the clinical application of Traditional Chinese Medicine.To be used in swelling and pain from carbuncles, falls, contusion and pain in Bisyndromes due to wind-damp.But its toxicity is evident,the dosage is often so low that can't show obvious effect in clinic use. Semen strychni pulveratum is a kind of fine powder by blending starch with grinding strychnos alkaloids.The qualities of semen and semen strychni pulveratum are mature relatively.The content of strychnine is so narrow that it is hard to fit in with the rules. In addition, the present Pharmacopoeia chromatographic methods for the determination of strychnine have many problems. Such as sodium heptanesulfonate is too expensive; the acidness of mobile phase is so strong that it is harm to chromatographic column greatly; the method is not fit for mostly Reversed-Phase HPLC column.so it is necessary to change the method.By taking strychnos alkaloids and semen strychni pulveratum as the research objects, this topic studies on semen strychni pulveratum through source, characters, identification, inspection the contentment systematically and then revise the quality standard of Semen strychni pulveratum.We make a method for the determination of BruNO and StryNO in semen strychni and its processing products; Build a new method for the determination of stry and bru; Microscopic identification and total ash determination are increased. Moreover, by using the HPLC method, there are some studies on fingerprinting of raw semen strychni and processed semen strychni in the essay.